To determine the genetic relationships among domestic dog breeds, we performed both a sequence comparison of mitochondrial DNA (mtDNA) and an amplified fragment length polymorphisms (AFLP) analysis. Three of four regions of mtDNA, cytochrome b, cytochrome oxidase subunit II, and 16S rRNA genes were highly homogeneous among dog breeds, whereas the other region, the control region, showed relativ...

High quality DNA was isolated from Casuarina equisetifolia which is widely planted in tropics and subtropics for its multiple utilities. Two commonly used methods, CTAB based and the commercially supplied DNeasyTM Plant Mini Kit (QIAGEN GmbH, Germany) were tested for their efficiency in isolating high quantity and quality DNA to be utilized for AFLP analysis. The CTAB based protocol was modifie...

Species of the genus Aquilegia are exceptionally diverse in their floral morphology and color, commonly known as columbine. They are widely planted ornamentals and are highly attractive for hummingbirds. However, little is known about their genetic diversity. We examined the genetic diversity of the species and cultivars using amplified fragment length polymorphism (AFLP) markers. Sixteen EcoRI...

The genetic diversity of 20 Iranian populations of Bunium persicum has been evaluated with random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers. Fresh leaves of seedlings from each population were used for genomic DNA extraction. Analysis of banding patterns of 15 RAPD primers and 17 AFLP primer combinations, revealed 192 (86%) and 228 (75%) polymorp...

The WRKY gene family represents an ancient and highly complex group of transcription factors involved in signal transduction pathways of numerous plant developmental processes and host defense response. Up to now, most WRKY proteins have been identified in a few angiosperm species. Identification of WRKY genes in a conifer species would facilitate a comprehensive understanding of the evolutiona...

We examined 154 Norwegian B. cereus and B. thuringiensis soil isolates (collected from five different locations), 8 B. cereus and 2 B. thuringiensis reference strains, and 2 Bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (AFLP). We employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fr...

The aflatoxin producer Aspergillus flavus and the koji mold Aspergillus oryzae are morphologically similar species that belong to the Aspergillus section Flavi. We used amplified fragment length polymorphism (AFLP) to differentiate these two species. In this study, we tested thirteen A. flavus, nine A. oryzae, and three A. flavus var. columnaris strains. DNA fragment profiles amplified with eac...

We examined 154 Norwegian B. cereus and B. thuringiensis soil isolates (collected from five different locations), 8 B. cereus and 2 B. thuringiensis reference strains, and 2 Bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (AFLP). We employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fr...

A genetic map for the model legume Lotus japonicus has been developed. The F(2) mapping population was established from an interspecific cross between L. japonicus and L. filicaulis. A high level of DNA polymorphism between these parents was the source of markers for linkage analysis and the map is based on a framework of amplified fragment length polymorphism (AFLP) markers. Additional markers...

Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of 14 strains of Xanthomonas arboricola pv. pruni and seven strains of X. axonopodis pv. phaseoli, which are used in xanthan production studies. Relationships identified by the AFLP profiles were assessed for xanthan production capacity, geographical location and host plant. Strains were isolated from 10 dif...