UNLABELLED BACKGROUND This study evaluated the adsorption capacity of the natural materials chitin and oyster shell powder (OSP) in the removal of saxitoxin (STX) from water. Simplified reactors of adsorption were prepared containing 200 mg of adsorbents and known concentrations of STX in solutions with pH 5.0 or 7.0, and these solutions were incubated at 25°C with an orbital shaker at 200 R...

Adsorption is a common unit operation in separation and purification of biotechnological products where chromatography steps can make up more than half the amount of the total purification costs [1]. The molecular mechanisms of adsorption are still not understood in detail. Further understanding of interactions between adsorbent surfaces and adsorptives could help to facilitate process design i...

Bacillus thuringiensis and recombinant Escherichia coli proteinaceous protoxins were subject to proteolysis and analyzed by capillary electrokinetic chromatography. Three resulting toxins (65 kDa) were baseline-resolved within 22 min using a 10 mM borate, pH 11 separation buffer consisting of 25 mM sodium dodecyl sulfate (SDS) and 30 mM phytic acid. The toxins displayed differential interaction...

A major problem in the measurement of serum bile acids is their quantitative extraction from the high molecular protein matrix. In our hands, the standard techniques of adsorption and reversed-phase chromatography have yielded incomplete recovery for different bile acids (33-93%) and poor reproducibility. In contrast, with the novel extraction procedure of size exclusion chromatography, recover...

The flavonoid phlorhizin is abundant in the leaves of Sweet Tea (ST, Lithocarpus Polystachyus Rehd). Phlorhizin was preparatively separated and purified from a crude ST extract containing 40% total flavonoids by static adsorption and dynamic desorption on ADS-7 macroporous resin and neutral alumina column chromatography. Only water and ethanol were used as solvents and eluants throughout the wh...

A procedure was developed to concentrate and partially purify porcine factor VIll:C from plasma by using conventional precipitation and chromatographic techniques. Blood from heparinized animal(s) was collected in citrate. soybean trypsin inhibitor. #{128}-aminocaproic acid and benzamidine. After aluminum hydroxide adsorption. polyethylene glycol-6000 precipatation. QAE-cellulose chromatography...

Combined gas-liquid chromatography mass-spectroscopy was used to identify lithocholic acid and Confirm the presence of other bile acids in serum of a healthy fasting subject. GLC was used to measure deoxycholic (DCA), chenodeoxycholic (CDCA), and cholic (CA) acids in sera. Before analysis, serum bile acids were purified by (a) enzymatic hydrolysis of conjugates, (b) anion-exchange chromatograph...

This paper aims to be an aid to those chemists who are interested in utilizing high-speed counter-current chromatography (HSCCC), which is free of irreversible adsorption and offers high resolution comparable to column chromatography. It explains the selection of HSCCC conditions step by step including the selection of two-phase solvent systems, determination of partition coefficient (K) of ana...

Coloradocin was isolated from a fermentation broth by adsorption onto Amberlite XAD-2. The activity was eluted in MeOH and purified by gel filtration on Shephadex LH-20, followed by liquid-liquid chromatography on diol-bonded silica gel. The last two steps in the purification of this antibiotic included reverse-phase chromatography on C18-bonded silica gel and countercurrent chromatography on a...

The effect of isotherm parameters of a displacer on the efficiency of desorption Chromatography has been investigated numerically. A general nonlinear multicomponent rate equation model with Langmuir isotherm was used in this study. It was found that the best displacer in this kind of operation is usually not the one that is more strongly adsorbed than the adsorbates when the operation is to di...