AIMS Use of the anti-tumour antibiotic actinomycin D is associated with development of hepatotoxicity, particularly in young children. A paucity of actinomycin D pharmacokinetic data make it challenging to develop a sound rationale for defining dosing regimens in younger patients. The study aim was to develop a physiologically based pharmacokinetic (PBPK) model using a combination of data from ...

Aryl hydrocarbon hydroxylase is a microsomal mixed function oxygenase induced by benz[a]anthracene and other polycyclic hydrocarbons in mammalian cell culture. Actinomycin D or Z-mercapto-1-(/3-4-pyridethyl)benzimidazole (inhibitors of RNA synthesis) or cycloheximide or puromycin (inhibitors of protein synthesis), added simultaneously with benz[a]anthracene, completely prevents aryl hydrocarbon...

The base compositions of nucleolar and extranucleolar nuclear IlNA's of the tumor and the liver were determined in controls and rats treated with actinomycin D in a dose of 150 Mg/kg of body weight. A decrease in content of cytosine and an increase in A + U/G + C ratio in the nucleolar RNA of the liver were found, but in the tumor the effects were not marked. Using ATP-U-32P2 or GTP-a-32P, the ...

Berry, L. Joe (Bryn Mawr College, Bryn Mawr, Pa.), Dorothy S. Smythe, and Louise S. Colwell. Inhibition of inducible liver enzymes by endotoxin and actinomycin D. J. Bacteriol. 92:107-115. 1966.-Bacterial endotoxin at the ld(50) level lowers liver tryptophan pyrrolase in mice, it prevents for 16 to 20 hr the induction of the enzyme by a concurrent injection of cortisone, it lowers significantly...

AIM To study the effects of actinomycin D on subcellular distribution of nucleolar protein fibrillarin in HEp-2 (human esophageal epithelial type 2) cells, and molecular mechanisms for maintenance of fibrillarin in nucleolus. METHODS Indirect immunofluorescence assay was employed to investigate subcellular distribution of nucleolar protein fibrillarin and immunoblotting analysis was used to d...

BACKGROUND Despite their broadly recommended use as chemotherapeutic agents, the porphyrogenicity of methotrexate and actinomycin D have not been confirmed. Accordingly, it is not known whether these agents are safe for use in patients with porphyria. CASE PRESENTATION In this report, we present a case of an invasive mole with lung metastasis in a 49-year-old Japanese woman who had previously...

Enhanced lethality for BALB/c mice has been observed after the administration of Salmonella typhosa endotoxin with either actinomycin D, cycloheximide, or nogalamycin. The dose of actinomycin D required to kill half of the mice (LD(50)) was 0.8 mg/kg in normal animals, 0.35 mg/kg in mice administered 0.08 mg of endotoxin per kg, and 0.28 mg/kg in mice administered 0.2 mg of endotoxin per kg. Th...