The photoreactions of 9-[6-(4-azidobenzamido)hexylamino]acridine (AHA) and 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) with double stranded DNA result in formation of single strand nicks and alkali labile sites (adducts) with an efficiency of 6 x 10(-3) nicks per AHA and 3 x 10(-2) nicks per DHA molecule. The alkali dependent DNA cleavage by AHA shows a pronounced A+G pre...

In the title compound, C(19)H(15)NO(4), the acridine system is essentially planar (r.m.s. deviation = 0.015 Å). The crystal packing exhibits π-π inter-actions between pairs of centrosymmetric mol-ecules, one of them between the central heterocyclic rings and others between the outer benzene rings of the acridine systems, with centroid-centroid distances of 3.692 (1) and 3.754 (1) Å, respectivel...

Two structurally similar compounds were found to inhibit sporulation in Bacillus subtilis 168. A dye, acridine orange, and an antischizophrenic drug, promethazine, blocked spore formation at concentrations subinhibitory to vegetative growth, while allowing synthesis of serine protease, antibiotic, and certain catabolite-repressed enzymes. The sporulation process was sensitive to promethazine th...

In the crystal structure of the title compound, C(19)H(14)N(+)·CF(3)SO(3) (-), the cations are linked to each other by very weak C-H⋯π inter-actions, while the cations and anions are connected by N-H⋯O, C-H⋯O and S-O⋯π inter-actions. The acridine ring system and the phenyl ring are oriented at an angle of 80.1 (1)° with respect to each other. The mean planes of adjacent acridine units are eithe...

'The uptake of vital dyes by lysosomes (1, 2) has permitted the microscopic observation of these subcellular organelles in living cells (3) and tissue homogenates (4) . Acridine orange (3 , 6-bis-dimethylaminoacridine), a fluorescent metachromatic dye, has been shown to concentrate within lysosomes, causing them to appear as bright orange granules in fluorescence microscopy (5-7) . We have obse...

The title ion pair, C(13)H(10)N(+)·C(6)H(3)N(2)O(4) (-), contains a protonated acridine cation and a 3-carb-oxy-pyrazine-2-carboxyl-ate monoanion, which are linked together through O-H⋯O, N-H⋯O and weak C-H⋯O hydrogen bonds. These hydrogen bonds generate a C(10) chain graph-set motif. The crystal structure is further stabilized by extensive π-π stacking inter-actions between nearly parallel [di...

with Acridine Orange By DAvID M. JOHNSON, PEYTON T. PIiArr AND PEnnY G. RuGBY I HE IMPORTANCE OF lymphocytic RNA in immune function has received widespread attention in recent years.’ A case in point is the work of Mannick and Egdahl showing that adoptive immunity can be transferred from one animal to another by means of lymphocytic RNA.2 The RNA of the lymphocyte can be evaluated and compared ...

The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. ...

Three-part differential white blood cell counts are used for disease diagnosis and monitoring at the point-of-care. A low-cost, miniature achromatic microscope was fabricated for identification of lymphocytes, monocytes, and granulocytes in samples of whole blood stained with acridine orange. The microscope was manufactured using rapid prototyping techniques of diamond turning and 3D printing a...

In the crystal structure of the title compound, C(23)H(20)NO(4) (+)·CF(3)SO(3) (-), the cations are linked through C-H⋯O, C-H⋯π and π-π inter-actions [centroid-centroid distances = 3.641 (2) and 3.885 (2) Å]. The cation and the anion are held together by C-H⋯O and S-O⋯π inter-actions. The acridine ring system and the benzene ring in the cation are oriented at a dihedral angle of 8.7 (1)°. The c...