A simple and sensitive chemiluminescence-based method was established for the determination of rabeprazole.The proposed method was based on the enhancing effect of rabeprazole on Ce(IV)-Na2SO3 -Tb(III) chemiluminescence reaction. A possible mechanism was discussed for chemiluminescence system by studying UV-Vis, fluorescence and chemiluminescence spectra. The effects of va...

There are two main methods for preparing the PuraMatrix (PM) as a three-dimensional (3-D) scaffold for supporting the cells including surface plating and encapsulation. In the surface-plating procedure, the scaffold is created by adding an ionic agent such as medium to PM. After adjusting the PH by changing the medium, cells are seeded on top of the scaffold. In the encapsulation method, PM and...

Chronic metabolic acidosis increases urinary calcium excretion without altering intestinal calcium absorption, suggesting that bone mineral is the source of the additional urinary calcium. In vivo and in vitro studies have shown that metabolic acidosis causes a loss of mineral calcium while buffering the additional hydrogen ions. Previously, we studied changes in femoral, midcortical ion concen...

Legumin from pea (Pisum sativum) is a molecule made up of six pairs of subunits, each pair consisting of an ;acidic' subunit (mol.wt. about 40000) and a ;basic' subunit (mol.wt. about 20000) linked by one or more disulphide bonds. The heterogeneity of legumin has been investigated by isoelectric focusing; undissociated legumin could not be focused satisfactorily, but legumin subunits could be a...

Uptake of L-[H]leucine by lobster hepatopancreatic brush-border membrane vesicles was stimulated by a transmembrane NaCl gradient (o>i ) , but not by identical gradients of NaSCN or other Cl~ salts (e.g. K, Li, NH4 , Cs or choline), suggesting that amino acid transfer depended upon both Na and Cl~. In NaCl medium at acidic pH, leucine uptake was largely electroneutral and unresponsive to a tran...