OBJECTIVES To determine the prevalence and mechanisms of resistance to fusidic acid in clinical isolates of Staphylococcus epidermidis. METHODS MICs of fusidic acid were determined for S. epidermidis isolates collected from the Leeds General Infirmary and from around Europe. Fusidic acid-resistant isolates were probed for the presence of the horizontally acquired resistance determinants fusB ...

INTRODUCTION .......................................................... 409 PURIFUICATION OF PLASMID DEOXYRIBONUCLEIC ACID FROM STREPTCOCCI ......................................................... 412 CONJUGATION AND PLASMID TRANSFER ......... ........................ 413 SEX PHEROMONES IN STREPTOCOCCUS FAECALIS ....... ................ 415 RESISTANCE TRANSFER IN THE ABSENCE OF PLASMID DEOXYRI...

background and objectives: shigella plays an important role as a causative organism of acute gastroenteritis, in children and others. rapid emergence of antibiotic resistance warrants continuous monitoring of susceptibility pattern of bacterial isolates. we report here our findings about shigella spp. isolates and their drug resistance patterns in nepalese patients. materials and methods: the s...

Populations maintain considerable segregating variation in the response to toxic, xenobiotic compounds. To identify variants associated with resistance to boric acid, a commonly-used household insecticide with a poorly understood mechanism of action, we assayed thousands of individuals from hundreds of strains. Using the Drosophila Synthetic Population Resource (DSPR), a multi-parental populati...

Background: Topical antimicrobial drugs are indicated for limited superficial pyodermitis treatment, although they are largely used as self-prescribed medication for a variety of inflammatory dermatoses, including atopic dermatitis. Monitoring bacterial susceptibility to these drugs is difficult, given the paucity of laboratory standardization. Objective: To evaluate the prevalence of Staphyl...

The induction of acid-shock proteins and the degree of acid resistance conferred on Clostridium perfringens by acid shock, and the kinetics of this resistance were determined. A sublethal acid shock at pH 4.5 for 20 min increased the acid tolerance of cells at least fifteenfold. The acquired tolerance was maintained for 3 h after acid treatment. The response of the microorganism to acid shock w...

Knowledge of the molecular genetic basis of resistance to antituberculous agents has advanced rapidly since we reviewed this topic 3 years ago. Virtually all isolates resistant to rifampin and related rifamycins have a mutation that alters the sequence of a 27-amino-acid region of the beta subunit of ribonucleic acid (RNA) polymerase. Resistance to isoniazid (INH) is more complex. Many resistan...