Almond emulsin peptide:N-glycosidase has been partially purified by using a new 3H-labeled 5-dimethylaminonaphthalene-1-sulfonyl-octaglycopeptide substrate derived from ovalbumin. The enzyme hydrolyzes the beta-aspartylglycosylamine linkage of both high mannose and biantennary complex glycopeptides, as shown by the isolation of the corresponding carbohydrate-free peptides containing aspartic ac...
