BACKGROUND AND AIMS Transgenics are used to demonstrate a causal relationship between ethylene insensitivity of a seedling legume plant, the level of ethylene receptor gene expression, lateral root growth and Mesorhizobium loti-induced nodule initiation. METHODS Lotus japonicus plants expressing the dominant etr1-1 allele of the Arabidopsis thaliana gene encoding a well-characterized mutated ...

Mature olive fruit that produced barely detectable levels of ethylene produced much greater quantities when they were briefly dipped in a solution containing 2 mM 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene. A single 10 s dip resulted in a transient increase in ethylene production that peaked 1 day after the application. When the fruits were dipped daily, th...

2,4-Dinitrophenol (DNP) and high temperature (35 to 40 C) are known to inhibit C(2)H(4) production in various plant tissues. The present study was made to determine the step in the C(2)H(4) biosynthetic pathway (methionine --> S-adenosylmethionine [SAM] --> 1-aminocyclopropane-1-carboxylic acid [ACC] --> C(2)H(4)) at which these treatments exert their inhibitory effect. In mung bean hypocotyls ...

The tightly regulated expression patterns of structural cell wall proteins in several plant species indicate that they play a crucial role in determining the extracellular matrix structure for specific cell types. We demonstrate that AtPRP3, a proline-rich cell wall protein in Arabidopsis, is expressed in root-hair-bearing epidermal cells at the root/shoot junction and within the root different...

Most dicotyledonous species respond to Fe deficiency by developing several mechanisms known as Fe-deficiency stress responses. To study the regulation of these responses, young cucumber plants (Cucumis sativus L. cv Ashley) were grown in nutrient solution for 11 d, being deprived of Fe during the last 4 or 5 d. Inhibitors of ethylene synthesis (2 or 10 [mu]M aminoethoxyvinylglycine; 10 or 20 [m...

Many soil bacteria contain 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, which degrades ACC, a precursor of the phytohormone ethylene. In order to examine the regulation of the acdS gene encoding ACC deaminase in Mesorhizobium loti MAFF303099 during symbiosis with the host legume Lotus japonicus, we introduced the beta-glucuronidase (GUS) gene into acdS so that GUS was expressed under ...

The main aim of the present study was the evaluation of proteins and antioxidant potential in ethylene-treated kiwifruit during the first 10 days of ripening. Kiwifruit samples were randomly divided into two groups: treated and untreated. Flesh firmness, sensory value, visual score, free sugars, soluble solids, ethylene biosynthesis, proteins, dietary fibers, total polyphenols and antioxidant p...

Shang Fa Yang was born in Taiwan in 1932. After receiving his B.S. and M.S. degrees in Agricultural Chemistry from the National Taiwan University, he came to the United States in 1958 to pursue a Ph.D. degree at Utah State University. Following three postdoctoral years, he was hired at the University of California, Davis, in 1966 where he was a biochemist and professor for 28 years. After retir...

Of nine plant growth regulators (indoleacetic acid, 1-naphthalene acetic acid, gibberellic acid, giberellin 4/7, 6-benzylaminopurine, 6-furfurylaminopurine, abscisic acid, and 1-aminocyclopropane carboxylic acid) tested, only 6-benzylaminopurine and abscisic acid affected (14)C-photosynthate unloading from excised seed coats of Phaseolus vulgaris L. Unloading, in the presence of KCl, was stimul...

Leaf senescence is one of the most limiting factors to plant productivity under salinity. Both the accumulation of specific toxic ions (e.g. Na+) and changes in leaf hormone relations are involved in the regulation of this process. Tomato plants (Solanum lycopersicum L. cv Moneymaker) were cultivated for 3 weeks under high salinity (100 mM NaCl) and leaf senescence-related parameters were studi...