Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting hazardous materials. Pollutants, such as heavy metals and chemicals, are now considered as a global threat and are associated with detrimental health outcomes. Fast and accurate detection of pollutants is essential to reduce t...

A validated method has been developed for the simultaneous measurement of reduced and oxidized glutathione in de-proteinised cellular extracts. This has been used to compare models of malignant and non-malignant human prostate cell lines. Analysis of LNCaP and DU145 cells showed a glutathione to glutathione disulfide ratio of 8:1 and 32:1 respectively, whilst the control cell line, PZ-HPV7 disp...

background and objective: in addition to the use of chemicals in food, pharmaceutical, cosmetic and hygiene industries, use of herbal and natural compounds have also increased. it seems natural ingredients compared with the chemical components show less side effects, such as mutagenicity and carcinogenesis however, their use can also be associated with side effects and toxicity. some significan...

Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting hazardous materials. Pollutants, such as heavy metals and chemicals, are now considered as a global threat and are associated with detrimental health outcomes. Fast and accurate detection of pollutants is essential to reduce t...

One of the major complications in cancer chemotherapy is the development of resistance, and cisplatin, as one of the important medicines in treatment regimens of different cancers is not excluded. One of the most described cellular defense mechanisms involved in resistance is glutathione (GSH) and in this study, the effects of cisplatin on the total intracellular GSH level (GSHi) in some sensit...

In this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in Iran. The cells were originating from two-day-old normal male calf of Holstein breed. The cell cultures were continuously passaged following complete proliferation of primary cells. The specific properties or characteristics of the cell were defined using cytogenet...

Background: Leukemia is a malignant proliferative disorder of the hematopoietic cells. The important role of angiogenesis in leukemia has been reported by several studies. Matrix metalloproteinases (MMPs) are a large group of endopeptidases which degredate the extracellular matrix and play an important role in angiogenesis. Objective: The present study was conducted to evaluate the patterns of ...

Neuropeptide Y (NPY) is a 36 amino acid peptide found throughout the central and peripheral nervoussystem of rat and human. NPY has been proposed to play an important role in satiety. The aim of this studywas to produce cell lines that secrete high levels of bioactive NPY. For this purpose, the complementaryDNA (cDNA) that encodes NPY was isolated by PCR. The cDNA was then clo...

Background: Interferons are some kind of natural cytokines which express in response to a variety of antigens including viral RNA, bacterial products, and tumor proteins. Interferon beta is used in the treatment of autoimmune diseases such as multiple sclerosis. Moreover, this drug inhibits cellular proliferation as well as angiogenesis and as a result, helps to cure cancer. In this research, i...

aim and background: hepatocellular carcinoma (hcc) -an aggressive malignancy with high rate of mortality- is the third cause of cancer related death, worldwide. cisplatin is a chemotherapy drug used for treatment of hcc. however, serious side effects of drug limit the therapeutic administer dose.  nanotechnology based devices such as liposome nanoparticles are capable to overcome these drawback...