A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI only stained degenerate cells that had lost their membrane integrity. Staining with SYBR-14 resulted in the nuclei of living sperm fluorescing bright green. Aliquots containing nearly all living bovin...

In view of the emerging importance of the ILs as "green" materials with wide applications and our general interests in green processes, a series of a twenty five new 1-alkyl-3-(4-phenoxybutyl) imidazolium-based ionic liquids (ILs) derivatives is synthesized using a facile and green ultrasound-assisted procedure. Their structures were characterized by FT-IR, 1H-NMR, 13C-NMR, 11B, 19F, 31P, and m...

Nitrogen is a crucial component that acts as building blocks for nucleic acids and proteins. It abundantly present in atmosphere of the earth but plants are not able to easily utilize it. Diazotrophic microorganisms such Rhizobium convert N2 make it available pulse crops. In study, total thirty-four isolates were recovered, from twenty-two with gram-negative-rods biochemically characterized. Mo...

L. amylophilus GV6 was studied for production of L(+) lactic acid in single step fermentation using starchy substrates. Seven types of inexpensive organic nitrogen supplements (flour of pigeon pea, red lentil gram, black gram, bengal gram, green gram, soya bean and baker’s yeast) were evaluated for their potential to replace more expensive commercial nitrogen sources, peptone and yeast extract....

To Doron Zeilberger, with our warmest regards, on the occasion of his sixtieth birthday. Abstract. The secant and tangent numbers are given (t, q)-analogs with an explicit com-binatorial interpretation. This extends, both analytically and combinatorially, the classical evaluations of the Eulerian and Roselle polynomials at t = −1.

This review presents an overview of silver nanoparticles (Ag NPs) preparation by green synthesis approaches that have advantages over conventional methods involving chemical agents associated with environmental toxicity. Green synthetic methods include mixed-valence polyoxometallates, polysaccharide, Tollens, irradiation, and biological. The mixed-valence polyoxometallates method was carried ou...

Doxycycline, a new generation tetracycline antibiotic, is currently one of choice for the treatment and prevention of infections caused by agents of biowarfare. We are developing real time PCR assays to detect tetracycline resistance genes in Gram-negative bacteria. The assay was developed as a multiplex SYBR Green I detection using the Roche Lightcycler and multi-melting peak analysis followed...