Homogenates prepared from the livers of rats treated for 3 days with the uncoupling agent 2,4-dinitrophenol contained considerably higher cyanide-sensitive superoxide dismutase activity when compared with homogenates of saline-treated animals. The effect was specific for only one form of the enzyme, however. Administration of 2,4-dinitrophenol at a dosage and on a schedule which produced the cl...

Introduction: Nitrophenol compounds are toxic compounds found in industrial wastewaters. 2,4-dinitrophenol is the most dangerous compound among phenolic compounds. The aim of this study was to evaluate the removal of 2,4-DNP from wastewater by microwaved dried sludge adsorbent. Materials and Methods: The results of 2,4-DNP removal were discontinuously obtained by the high performance liquid ch...

A series of experiments was performed to test the proposal of others that the oxidation of ethanol to acetate is NADflinked, and that, when transferred into the mitochondria, the cytoplasmic reducing equivalents produced during ethanol oxidation inhibit the oxidation of glucogenic precursors required for the formation of P-enolpyruvate. Particulate-free dialyzed extracts of rabbit livers oxidiz...

The experiments described previously (Birt & Bartley, 1960a, b) have shown that the amounts and state of reduction of the endogenous pyridine nucleotides of rat-liver mitochondria are controlled by the components of the incubating medium. This paper is concerned with a general study of the effects of the temperature of incubation, the addition of various substrates, 2:4-dinitrophenol and amytal...

This paper describes experiments on the effect of the oxidation-reduction state of the respiratory carriers in fragments of mitochondria on three partial or intermediate reactions of the oxidative phosphorylation mechanism which occur in the absence of net electron transport, namely the adenosine 5’-triphosphateinorganic Pa2 exchange reaction (I), the ATPase and dinitrophenol-stimulated ATPase ...

Antiviral activity on Newcastle disease virus was examined with some respiration inhibitors including ascochlorin, rotenone, antimycin A(3), piericidin A, dicoumarol, 2, 4-dinitrophenol, pentachlorophenol, and fatty acids. Of the chemicals tested, ascochlorin and rotenone showed significant inhibitory effect on the viral growth in cultured cells as determined by the plate and tube assay methods...

DRUGS AND UPTAKE OF 32p BY ERYTHROCYTES 533' 5. Sodium salicylate and sodium thiopentone depressed the 32p uptake of 2:3-diphosphoglycerate and adenosine triphosphate to a greater extent than they depressed the 32P exchange of inorganic phosphate and hexose monophosphate. These agents do not depress 32p uptake by inhibiting triose phosphate dehydrogenase, for neither drug had any effect on glyc...

A semisynthetic antibody library composed of single chain Fv fragments (scFv) was constructed by replacing the heavy chain CDR3 region of a human scFv by a random sequence of eight amino acids using trinucleotide codons. After cloning into a phage display vector, an antibody library was generated with a complexity of 8 x 10(8) independent clones. The library was screened for binders to dinitrop...