When BALB/c 3T3, simian virus 40 (SV40)-transformed 3T3 (SVT2), and revertant variants of the transformed cells are removed by EGTA treatment from the substrate on which they were grown, they leave behind a layer of glycoprotein which has been characterized biochemically (Terry, A. H. and L. A. Culp. 1974. Biochemistry. 13:414.)-substrate-attached material (SAM). The influence of SAM from norma...

Tumor necrosis factor (TNF), a cytokine, and okadaic acid, a tumor promoter, strongly phosphorylated the same proteins, vimentin and heat shock protein 27, although their time courses were different. Human TNF-a at a concentration of 0.6 UMmarkedly stimulated transformation of BALB/3T3 cells initiated with 3-methylcholanthrene. The human TNF-a was about 1000 times more effective than the chemic...

Phosphoinositide-specific phospholipase Cgamma (PLCgamma) is a key regulatory enzyme that binds to the phosphoryl-tyrosine residues in the cytoplasmic domain of certain activated receptors and catalyses the hydrolysis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] forming IP3 and diacylglycerol (DAG) in response to several mitogenic factors. Previously, we determined that microinjecte...

The extracts of Eriobotrta japonica leaves with the 3H-glucose uptaking activity in 3T3-L1 adipocytes were separated by TLC for two times. On the basis of UV-vis spectral, NMR and MS data, corosolic acid was identified as activity components. Moreover, the effects of corosolic acid on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes was studied. The results showed that 3H-glucos...

Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenes...

The BALB/c 3T3 NRU test is probably the cytotoxicity test that has been used most frequently in formal validation programs, all of which were aimed at evaluation of cytotoxicity in predicting eye irritancy. Large-scale studies to be mentioned here are Phases I, II, and III of the Cosmetic, Toiletry, and Fragrance Association (CTFA) validation program (Gettings et al., 1991, 1992, 1994a, 1994b);...

Using a subtraction method, we have isolated genes that are induced early in the differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. These include the genes encoding transcription factors and signalling proteins, as well as unknown genes. Bach1, a transcription factor, and ARA70, a cofactor, were rapidly induced during differentiation. The induction of these two genes was observ...

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The level of the neutral glycolipid, Galnac beta 1 leads to 4 Gal beta 1 leads to 4Glc leads to Cer (asialo GM2), in BALB/c 3T3 mouse fibroblasts transformed by Kirsten murine sarcoma virus (3T3KIMSV) was greatly increased compared to the nontransformed parental cells (3T3). This elevated chemical quantity was found to be localized on the surface of intact cells and accessible to external reage...