Stereoselectively labelled isotopomers of NAD(P)H are highly relevant for mechanistic studies of enzymes which utilize them as redox equivalents. Whereas several methods are firmly established for their generation in high diastereomeric purity by enzymatic methods, alternative methods have so far not been investigated. The article presents the stereoselective deuteration of NAD at the 4-positio...

OBJECTIVES To investigate the effect of three cycling cadences on a subsequent 3000 m track running performance in well trained triathletes. METHODS Nine triathletes completed a maximal cycling test, three cycle-run succession sessions (20 minutes of cycling + a 3000 m run) in random order, and one isolated run (3000 m). During the cycling bout of the cycle-run sessions, subjects had to maint...

The objective of this study was to determine the roughage to concentrate (R:C) ratio with rain tree pod meal (RPM) supplementation on in vitro fermentation using gas production technique. The experiment design was a 6×4 factorial arrangement in a CRD. Factor A was 6 levels of R:C ratio (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) and factor B was 4 levels of RPM (0, 4, 8 and 12 mg). It was fou...

during storage, serum separation is a major defect that affects the market share of doogh. therefore, in the present study the effects of high shear stirring and homogenization, as major operation units, on the stability was investigated. to do so, dooghs were stirred at various speeds and time scales (9000 rpm and 11000 rpm for 30 s and 2 min), homogenized at different pressures (0, 20, 50, 80...

We hypothesized that a higher pedal rate (assumed to result in a greater proportional contribution of type II motor units) would be associated with an increased amplitude of the O(2) uptake (Vo(2)) slow component during heavy-cycle exercise. Ten subjects (mean +/- SD, age 26 +/- 4 yr, body mass 71.5 +/- 7.9 kg) completed a series of square-wave transitions to heavy exercise at pedal rates of 35...

The cystolic sulfotransferse 1C3 (SULT1C3) gene was identified by computational analysis of the human genome and suggested to contain duplications of its last two exons (7a/b and 8a/b). Although the SULT1C3 isoform containing the more downstream exons 7b and 8b (SULT1C3d) has been expressed in Escherichia coli, crystallized, and characterized for activity, there is currently no evidence that SU...

BACKGROUND Alkaline amylase has significant potential for applications in the textile, paper and detergent industries, however, low yield of which cannot meet the requirement of industrial application. In this work, a novel ARTP mutagenesis-screening method and fermentation optimization strategies were used to significantly improve the expression level of recombinant alkaline amylase in B. subt...

The nanocrystalline hydroxyfluorapatite (HFA) was synthesized by mechanosynthesis with a planetary ball mill (PM200). disc to jar speed ratio constant (equal 0.5). effect of different milling parameters such as grinding duration, balls number’s, initial powder mass and rotation were studied apprehend their effects on both the process grinding/mechanosynthesis synthesis HFA. Unlike previous stud...