kawasaki disease (kd) is an inflammatory multiorgan disease of unknown etiology. the most dramatic organ involved is the heart. there were a few studies about cardiac involvement and sterile pyuria. this study guides to determine if sterile pyuria is associated with coronary artery aneurysm (caa) in kd patients and to consider it as a predicting factor for coronary artery involvement. forty sev...

To test the hypothesis that the abundance of the apical urea transporter of the inner medullary collecting duct (IMCD) is regulated in vivo by factors associated with altered water balance, immunoblots of rat inner medullary membrane fractions were probed with rabbit polyclonal antibodies against the renal urea transporter (RUT) gene product. In inner medullas of Brattleboro rats, which manifes...

We have cloned part of the genes encoding two antigens of 71 and 72 Kd froi P. falciparua (1,2). These polypeptides differ in their cellular location, expression during the parasite's erythrocytic cycle , isoeletric point in bi-dimensiona 1 gel electrophoresis and reaction with rabbit immune sera (1-3). The sequence of the 72 Kd gene identified it as a member of the P. falciparua heat-shock fam...

one of the emerging therapeutic strategies for targeted treatment of most cancers is the use of immunotoxins which are fusion proteins consisted of a targeting and a toxic moieties. we previously showed that the recombinant a254-gmcsf fusion protein selectively kills acute myeloblastic leukemia cells which harbor a large number of granulocyte-macrophage colony stimulating factor (gmcsf) recepto...

Phosphatidylinositol-3-kinase gamma (PI3Kγ) is a leukocyte-specific lipid kinase with signaling function downstream of G protein-coupled receptors to regulate cell trafficking, but its role in T cells remains unclear. To investigate the requirement of PI3Kγ kinase activity in T-cell function, we studied T cells from PI3Kγ kinase-dead knock-in (PI3Kγ(KD/KD)) mice expressing the kinase-inactive P...

Abstract Introduction Antibody characterisation is fundamental in transplantation and infectious diseases, but current immunoassays cannot determine two antibody properties, affinity (KD) concentration ([Ab]). We aimed to overcome these limitations allow in-depth profiling of antibodies directly sera provide insights into clinical translation. Methods Using a microfluidic diffusional sizing-bas...

This laboratory has been characterizing protein serine/threonine kinase reactions of hematopoietic tissues, whose most distinguishing characteristics in vitro are stimulation with vesicular phosphatidyl glycerol, and the ability to function using Mn2+ as the sole divalent cation. The major protein substrates are a 73-kD protein and a protein migrating near ovalbumin on sodium dodecyl sulfate po...

The biosynthesis, processing, and ligand-binding function of the membrane-associated and soluble forms of the folate-binding protein (FBP) in KB cells, a cultured human cell line, were studied using pulse-chase labeling with [35S] methionine. The intermediary and mature forms of the protein were isolated by immunoprecipitation and affinity chromatography and analyzed by sodium dodecyl sulfate e...

The effect of salt stress on the incorporation of [(35)S]methionine into protein was examined in roots of barley (Hordeum vulgare L. cv California Mariout 72). Plants were grown in nutrient solution with or without 200 millimolar NaCl. Roots of intact plants were labeled in vivo and proteins were extracted and analyzed by fluorography of two-dimensional gels. Although the protein patterns for c...