Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 µg 14D9ml-1. intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the cul...

Abstract The present study uses a wavelet-based clustering technique to identify spatially homogeneous clusters of groundwater quantity and quality data select the most effective input for feed-forward neural network (FFNN) model predict level (GL), pH HCO3? in groundwater. In second stage this methodology, first, GL, time series different piezometers were de-noised using threshold-based wavele...

We prove that for an irreducible representation τ : GL(n) → GL(W ), the associated homogeneousP k -vector bundle Wτ is strongly semistable when restricted to any smooth quadric or to any smooth cubic in P k , where k is an algebraically closed field of characteristic 6= 2, 3 respectively. In particular Wτ is semistable when restricted to general hypersurfaces of degree ≥ 2 and is strongly semis...

BACKGROUND Epidemiologic studies suggest that diets with a low glycemic index (GI) or glycemic load (GL) are associated with a decreased risk of developing metabolic syndrome. Intervention studies are inconsistent, however, possibly due to differences in macronutrient and fiber compositions of the experimental diets. OBJECTIVE We tested side-by-side test foods with similar macronutrient and f...

Two pure bacterial strains capable of rapid degrading methyl tert–butyl ether (MTBE) were isolated from an industrial wastewater treatment plant, identified and characterized. These strains are able to grow on MTBE as the sole carbon and energy sources and completely mineralize it to the biomass and carbon dioxide. The strains were identified as Bacillus cereus and Klebsiella terrigena. Bot...

OBJECTIVE To assess the ability of a food-frequency questionnaire (FFQ) to rank Australians according to their intake of total carbohydrate, sugar, starch, fibre, glycaemic index (GI) and glycaemic load (GL). DESIGN Cross-sectional sample from a population cohort. SETTING Two postcode areas west of Sydney, Australia. SUBJECTS From 1992 to 1994, a total of 2868 older Australians provided d...

Background: There is no clear data on the adherence of emergency medical services (EMS) paramedics and hospital staff rather than those working in poisoning centers to the guidelines for managing acutely poisoned patients in developing countries. Methods: During a 6-month period, all EMS-managed poisoned patients along with those initially managed in a non-poisoning center before being refer...

Segregation of the germ line from the soma is an essential event for transmission of genetic information across generations in all sexually reproducing animals. Although some well-studied systems such as Drosophila and Xenopus usematernally inherited germ determinants to specify germ cells, most animals, including mice, appear to utilize zygotic inductive cell signals to specify germ cells duri...