The coronavirus disease 2019 (COVID-19) pandemic has focused attention on the need to develop effective therapeutics against causative pathogen, severe acute respiratory syndrome 2 (SARS-CoV-2), and also other pathogenic coronaviruses. In this study, we report a kind of bisbenzylisoquinoline alkaloid, neferine, as pan-coronavirus entry inhibitor. Neferine effectively protected HEK293/hACE2 HuH7...