Complexes 11, 22 and 33 were synthetized according to adapted published protocols. Tris(3,3′,3′′-phosphinidynetris(benzenesulfonato)palladium(0) nonasodium salt nonahydrate] (complex 4), bathophenanthrolinedisulfonic acid disodium salt , bathocuproin disulfonic acid disodium, 2,2′-Biquinoline-4,4′-dicarboxylic acid dipotassium salt, triphenylphosphine-3,3′,3′′-trisulfonic acid trisodium salt li...

In the guinea-pig renal pelvis, most smooth muscle cells examined (>90%), using a conventional microelectrode, had a resting membrane potential of about -50 mV and produced spontaneous action potentials with initial fast spikes and following plateau potentials. The remainder (<10%) had a resting membrane potential of about -40 mV and produced periodical depolarization with slow rising and falli...

Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and its nuclear homolog CaMKP-N (PPM1E) are Ser/Thr protein phosphatases that belong to the PPM family. CaMKP-N is expressed in the brain and undergoes proteolytic processing to yield a C-terminally truncated form. The physiological significance of this processing, however, is not fully understood. Using a wheat-embryo cell-fr...

Sulfonic aromatic compounds are widely used in the textile industry for the synthesis of azo dyes. The presence of the sulfonic group endows these compounds with high water-solubility and resistance to biological treatment. The present work analysed the efficacy of oxidation with ozone in the treatment of waters containing naphthalene-1-sulfonic acid, naphthalene-1,5-disulfonic acid and naphtha...

The amino-reactive reagent, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS),(1) considerably reduces the uptake of the sulfhydryl agent, parachloromercuriphenylsulfonic acid (PCMBS), but does not reduce its effects on cation permeability and on cation transport. These data indicate that PCMBS enters the membrane by at least two channels, one sensitive and the other insensitive t...

Methylmercury (MeHg; 10-100 microM) induced a spontaneous, transient, slow inward current in Purkinje cells in rat cerebellar slices. Insensitivity of this current to tetrodotoxin suggests that its generation is not related to presynaptic firing. The present study was designed to attempt to identify the ionic origin of this current. Neither Gd(3+), a nonspecific cation channel blocker, nor tetr...

Calcitonin is known to stimulate Ca2+ reabsorption and natriuresis and to increase adenosine 3',5'-cyclic monophosphate levels in early distal tubule, but its effects on acid-base transport mechanisms are not well characterized in this segment. We found that recovery of cell pH (pH1) from an induced acid load (using NH4+) in single isolated segments of the initial portion ("bright") of the rabb...

Therapeutic proteins are among the most widely prescribed medications, with wide distribution and complex supply chains. Shipping exposes protein formulations to stresses that can trigger aggregation, although exact mechanism(s) responsible for aggregation unknown. To better understand how shipping causes we compared populations of aggregates were formed in a polyclonal antibody formulation dur...