teria and their relatives,” are a major class of bacteria (Gupta, 2000; Holt et al., 1994; Stackebrandt et al., 1988; Zinder, 1998). This group of predominantly Gram-negative bacteria contains more than 200 genera with a great diversity of phenotypic and physiological attributes. Proteobacteria have been classified based on homology of 16S ribosomal RNA or by hybridization of ribosomal RNA or D...

16S rRNA genes and transcripts of Acidobacteria were investigated in 57 grassland and forest soils of three different geographic regions. Acidobacteria contributed 9-31% of bacterial 16S rRNA genes whereas the relative abundances of the respective transcripts were 4-16%. The specific cellular 16S rRNA content (determined as molar ratio of rRNA : rRNA genes) ranged between 3 and 80, indicating a...

This paper reports the first isolation and culture of Ehrlichia canis in Spain from a naturally infected dog using the DH82 cell line. After DNA extraction and PCR amplification, a nearly complete (1412bp) sequence of the 16S rRNA gene of the new E. canis strain was obtained. The GenBank accession number for the nucleotide sequence of this strain is AY394465. This sequence was aligned with the ...

The bacterial 16S rRNA genes from blood samples of two patients with human ehrlichiosis and from an isolate recovered from one of the patients were amplified by using the polymerase chain reaction. The amplimers were then cloned and sequenced. The 16S rRNA gene sequence was also determined for Ehrlichia canis (two strains), E. equi, E. phagocytophila (two strains), and E. sennetsu (two strains)...

In the present investigation the diversity of the Staphylococcus species in different street vend food samples was studied. A total of 35 staphylococcal food isolates comprising of various species from 14 different street vend food samples were identified and characterized phenotypically. Staphylococcus aureus was found to be the most prevalent species in these foods. A PCR-RFLP analysis based ...

The present study deals with isolation, identification and analysis of bacteria from tannery effluent through 16S rRNA based molecular technique. Bacterial strain was isolated and characterized using various biochemical tests and confirmed through molecular approach. Bacterial 16S rRNA gene was amplified using suitable primers. The amplified 16S rRNA gene sequence was compared with the sequence...

Direct sequencing of environmental DNA (metagenomics) has a great potential for describing the 16S rRNA gene diversity of microbial communities. However current approaches using this 16S rRNA gene information to describe community diversity suffer from low taxonomic resolution or chimera problems. Here we describe a new strategy that involves stringent assembly and data filtering to reconstruct...

pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. a polymerase chain reaction (pcr) assay was developed using primers derived from conserved part of 16s-23s rrna gene. the pcr amplified a fragment size of 0.7 kb using dna from nine avian p. multocida  isolates...

The coding region for the Pneumocystis carinii small subunit rRNA (16S-like rRNA) contains 390 extra base pairs in a region that is highly conserved in other eukaryotic rRNA genes. The sequence has all the elements characteristic of group I self-splicing introns and interrupts the phylogenetically conserved helix proximal to the 3' terminus of the 16S-like rRNA. In vitro transcripts containing ...