AIMS A new diagnostic strategy to improve the detection of pathogens in heart valves (HVs) from patients with infective endocarditis (IE) was evaluated. METHODS AND RESULTS Three hundred and fifty seven HVs surgically removed from 326 patients with proven IE or suspicious intra-operative findings, examined by 16S rDNA polymerase chain reaction (PCR) and culture were retrospectively analysed a...

This study aimed to compare the occurrence and level of Bacillus cereus sensu stricto (B. cereus) in chili, previously isolated white pepper from traditional supermarkets around Bogor, determine phylogenetic relationship between obtained isolates based on their 16S rDNA gene, potential toxicity ces nheA genes using Polymerase Chain Reaction. The highest presumptive B. samples was 5.95×105 2.6×1...

A cluster of marine bacteria within the alpha-3 subclass of the class Proteobacteria accounted for up to 28% of the 16S ribosomal DNA (rDNA) sequences in seawater samples from the coast of the southeastern United States. Two independent oligonucleotide probes targeting 16S rDNA of this "marine alpha" cluster indicate that the group dominates bacterioplankton communities in estuarine and nearsho...

A cluster of marine bacteria within the a-3 subclass of the class Proteobacteria accounted for up to 28% of the 16S ribosomal DNA (rDNA) sequences in seawater samples from the coast of the southeastern United States. Two independent oligonucleotide probes targeting 16S rDNA of this “marine alpha” cluster indicate that the group dominates bacterioplankton communities in estuarine and nearshore r...

OBJECTIVE To evaluate broad-range 16S ribosomal DNA (rDNA) polymerase chain reaction (PCR) as a rapid screening tool to detect bacterial contamination of stem-cell products. METHODS We performed the evaluation using whole blood spiked with serially diluted bacterial-type strains. Detection sensitivity was defined as the bacterial concentration for which all replicates were positive at each co...

Phylogenetic trees can reveal the origins of endosymbiotic lineages of bacteria and detect patterns of co-evolution with their hosts. Although taxon sampling can greatly affect phylogenetic and co-evolutionary inference, most hypotheses of endosymbiont relationships are based on few available bacterial sequences. Here we examined how different sampling strategies of Gammaproteobacteria sequence...

Although diverse natural products have been isolated from the benthic, filamentous cyanobacterium Lyngbya majuscula, it is unclear whether this chemical variation can be used to establish taxonomic relationships among disparate collections. We compared morphological characteristics, secondary-metabolite compositions, and partial 16S ribosomal DNA (rDNA) sequences among several collections of L....

AIMS Desmodia are leguminous plants used as important forage and herbal medicine in China. Little information is available about the nodule bacteria of Desmodium species. To understand the genetic diversity of rhizobia associated with Desmodium species grown in China, isolates from temperate and subtropical regions were obtained and analysed. METHODS AND RESULTS A total of 39 rhizobial strain...

The present study dealt with the identification of a bacterium having dominance in earthworm (Lennogaster pusillus) midden by 16S rDNA based genomic analysis. The bacterium identified was Aeromonas punctata strain JM10 (Genbank Accession No: GU205197.1). Isolated DNA of the bacterium showed amplicon band of 1500 bp, when resolved on agarose gel. Amplification of the 16S rDNA gene from the Aerom...