Gene electrotransfer (GET) is a widely used method for nucleic acids’ delivery into cells. We explored, evaluated, and demonstrated the potential use of different pulse durations introducing plasmid DNA (pDNA) cells in vitro compared efficiency dynamics transgene expression after GET. performed experiments on cell suspensions 1306 fibroblasts C2C12 myoblasts with four ranges (nanosecond, high f...

In this research, the metal-organic framework of HKUST-1 (Hong Koung University of Science and Technology) was synthesized for use in modern drug delivery systems by the thermal solvent method. It was activated in two conditions: under vacuum pressure and by a freeze-drying method. The synthesized HKUST-1 Metal-Organic Framework was analyzed by IR, XRD, BET, and SEM. In order to examine and com...

The fluctuating property of the heat source is a technical obstacle waste recovery, which leads to part-load operation and reduced economics. This work presents novel system mitigate fluctuations by using steam-organic Rankine cycles (RC-ORC) two-stage steam accumulators. can switch between isothermal storage discharge simply regulation water mass flow (m2) from low-temperature accumulator (LTA...

Introducción: La capacidad es la aptitud que tiene persona para poder celebrar negocios jurídicos a partir del ejercicio de una voluntad reflexiva. En Colombia, Ley 1996 2019, regula un nuevo régimen el legal las personas con discapacidad mayores edad. Objetivo: Se aborda paradigma en situación Código Civil, pasando por reforma 1306 2009; y se describe comparativamente derogatorias, modificacio...

BACKGROUND Matrix metalloproteinase -2 (gelatinase-A, Mr 72,000 type IV collagenase, MMP-2) and -9 (gelatinase-B, Mr 92,000 type IV collagenase, MMP-9) are key molecules that play roles in tumor growth, invasion, tissue remodeling, metastasis and stem-cell regulation by digesting extracellular matrix barriers. MMP-2 and -9 are well known to impact on solid cancer susceptibility, whereas, in hem...