نتایج جستجو برای: 10 selected rapd primers produced 160 bands

تعداد نتایج: 1694252  

Journal: :Memorias do Instituto Oswaldo Cruz 2002
R G M Spada D da Silva A-Z Abdel-Hamid S S Sobral-Hamaguchi N R B Zuim E M Zanotti-Magalhaes L A Magalhaes J T Ribeiro-Paes

The analysis of the genetic variability related to susceptibility to Schistosoma mansoni infection in the vector of the genus Biomphalaria is important in terms of a better understanding of the epidemiology of schistosomiasis itself, the possible pathological implications of this interaction in vertebrate hosts, and the formulation of new strategies and approaches for disease control. In the pr...

2015
Ibrahim M. AlRawashdeh

Nine Random Amplified Polymorphic DNA (RAPD) primers showing polymorphic bands were used to determine the genetic relatedness among and within single individual plants of Lupinus pilosus and used for the construction of the dendrogram and the similarity matrix. A total of 1112 bands were obtained, 219 of which were polymorphic. Similarity values among the studied single individuals of Lupinus p...

Assessment of genetic diversity and individual relationships in rice (Oryza sativa) germplasm collections seems to be necessary for future rice breeding program. In order to understand genetic relationships of 30 rice genotypes, nine morphological traits, seven physicochemical properties and twelve RAPD primers were used for study of 30 rice genotypes. Among morphological traits, numbe...

Journal: :The Journal of veterinary medical science 2006
Ji-Dong Jin Dong-Seok Lee Eun-Kyung Shin Sun-Joong Kim Rose Jung Tae-Wook Hahn

Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initial...

Journal: :Genome 2000
V Subramanian S Gurtu R C Nageswara Rao S N Nigam

Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiol...

2009
Sureeporn Kate-ngam

Random amplified polymorphic DNA (RAPD) is a simple, fast and cost effective method for assessing genetic diversity of plant varieties. The reproducibility of the RAPD amplification was affected by several factors, therefore causing misinterpretation. In this study, we analyzed the effect of changing the concentration of magnesium chloride, template genomic DNA and Taq DNA polymerase with the o...

2009
Shamsun Naima Rahman Md Shahidul Islam Md Samsul Alam

Random Amplified Polymorphic DNA (RAPD) assay was performed to estimate genetic polymorphism in six different rice (Oryza sativa L.) cultivars viz. Basmati 370, DM 25, IRATOM 24, Binadhan 6, TNDB 100 and Y 1281. Three out of the 15 decamer random primers showed amplification of genomic DNA in 24 individuals. The primers produced a total of 26 bands of which 14 were polymorphic. Proportion of po...

Journal: :The Journal of heredity 2005
A K Shasany M P Darokar S Dhawan A K Gupta S Gupta A K Shukla N K Patra S P S Khanuja

Three controlled crosses were carried out involving Mentha arvensis and Mentha spicata [M. spicata CIMAP/C30 x M. spicata CIMAP/C33 (cv. Neera); M. arvensis CIMAP/C18 x CIMAP/C17 (cv. Kalka); and M. arvensis CIMAP/C17 x M. spicata CIMAP/C33]. The parents were subjected to random amplified polymorphic DNA (RAPD) analysis with 80 primers, and polymorphic primers were tested for detecting coinheri...

2010
S. Das R. C. Misra

Genetic diversity among fifteen finger millet genotypes was estimated using RAPD markers. Out of the initial twenty-five random primers, nine RAPD primers were found to be highly reproducible and produced a total of 60 loci of which 51 loci were polymorphic. Primer OPC18 amplified highest number of polymorphic bands. Percentage of polymorphism varied from 50% to 100% with an average of 80.8%. P...

Journal: :Sabrao Journal of Breeding and Genetics 2023

The presented research, comprising characterization of nine species the wild rose (Rosa L.), came from various regions Northern Iraq. study proceeded in 2021–2022 at College Education Pure Science, University Mosul, Employing random amplification polymorphism DNA (RAPD) helped determine genetic variation relationships among using statistical program Numerical Taxonomy and Multivariate Analysis ...

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