We used confocal microscopy and quantitative image analysis to follow the movement of the fluorescent organic anion fluorescein (FL) from bath to cell and cell to blood vessel in intact rat lateral choroid plexus. FL accumulation in epithelial cells and underlying vessels was rapid, concentrative, and reduced by other organic anions. At steady state, cell fluorescence exceeded bath fluorescence...

In the present work, we describe for the first time the specific role of cutinase on surface modification of cellulose acetate fibers. Cutinase exhibits acetyl esterase activity on diacetate and triacetate of 0.010 U and 0.007 U, respectively. An increase on the hydroxyl groups at the fiber surface of 25% for diacetate and 317% for triacetate, after a 24 h treatment, is estimated by an indirect...

The vital staining properties of rose cultures (Rosa cv Paul's Scarlet) of increasing age were compared with their ability to be subcultured. At 4-day intervals beginning on day 14, after cell division and expansion had stopped, cells were stained separately with Evans blue, fluorescein diacetate, and phenosafranine. The degree to which parent cultures stained with each of these dyes was compar...

In the title compound, [Cd(C(10)H(8)O(6))(C(10)H(8)N(2))(H(2)O)(2)](n), the Cd(II) ion has inversion symmetry and is coordinated by O atoms from two water mol-ecules and two bridging 2,2'-(μ-p-phenyl-enedi-oxy)diacetate ligands and two N atoms from two 4,4'-bipyridine ligands, giving a slightly distorted octa-hedral geometry. The diacetate and 4,4'-bipyridine ligands also lie across inversion c...

We recently developed a collagen vitrigel membrane (CVM) chamber possessing a scaffold composed of high-density collagen fibrils. In this study, we first confirmed that the advantage of CVM chamber in comparison to the traditional culture chamber with porous polyethylene terephthalate membrane is to preserve a culture medium poured in its inside even though the under side is not a liquid phase ...

The effect of two antimicrobials in the enhancement solution was investigated in this study. Sensory and shelf-life characteristics were measured for beef top rounds enhanced to 110% with 0.5% sodium chloride and 0.4% sodium tripolyphosphate (CNT); CNT with a 1% solution of 80% sodium citrate plus 20% sodium diacetate (SC + D); or CNT with 2% buffered vinegar (VIN) in the final product. SC + D ...

Listeria monocytogenes, a psychrotrophic foodborne pathogen, is an occasional postprocess contaminant on ready-to-eat meat (RTE) products including frankfurters. Ultraviolet C light (UVC) is an FDA-approved technology for the decontamination of food surfaces. In this study, the ability of UVC to inactivate L. monocytogenes on frankfurters that contained potassium lactate (PL) and sodium diaceta...

In situ drug and organic electrolyte complex tablets were investigated as extended release dosage forms. Incorporating a 1:1 molar ratio of diltiazem HCl and an anionic organic electrolyte (i.e., Na deoxycholate) into HPC tablets extended the total release time with a near zero – order release rate (release exponent, n = 0.85 – 0.97) due to in situ complex formation of the drug and the organic ...

Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2', 7'-dichlorodihydrofluorescein diacetate). Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable ...