Occlusive thrombosis depends on the net balance between platelets, coagulation, and fibrinolytic factors. Epidemiologic information suggests that plasminogen activator inhibitor-1 (PAI-1), a central regulator of the fibrinolytic system, plays an important role in determining the overall risk for clinically significant vascular thrombosis. Vitronectin (VN), an abundant plasma and matrix glycopro...

The low-density-lipoprotein-receptor (LDLR)-related protein (LRP) is composed of several classes of domains, including complement-type repeats (CR), which occur in clusters that contain binding sites for a multitude of different ligands. Each approximately 40-residue CR domain contains three conserved disulphide linkages and an octahedral Ca(2+) cage. LRP is a scavenging receptor for ligands fr...

Traditional Chinese medicine has been used to treat a variety of human diseases for many centuries. Zanthoxylum nitidum var. tomentosum is used as an adjuvant to promote blood circulation and remove stasis. However, the mechanisms of improving circulation and other biological activities of Z. nitidum var. tomentosum are still unclear. Plasminogen activator inhibitor-1 (PAI-1) regulates the plas...

A diacid monomer was synthesized by the condensation of L-tryptophan amino acid and pyromellitic dianhydride (PMDA). The diacid was utilized for the synthesis of three types of poly(amide-imide)s (PAIs) using three different kinds of diamines. The synthesized monomer and PAIs were characterized using FT-IR, 1H-NMR and 13C-NMR spectroscopies and the PAIs were also used ...

background: recurrent pregnancy loss (rpl) defined by two or more failed pregnancies before 20 weeks of gestation. several factors play a role in rpl including thrombophilic conditions which can be influenced by gene polymorphisms. plasminogen activator inhibitor-1 (pai-1) and angiotensin converting enzyme (ace) genes are closely related to fibrinolytic process, embryonic development and pregna...

change, although active PAI-1 changed to latent PAI-1 as a result of freezing and thawing. Although it is thought that measuring active PAI-1 concentrations is the best way to evaluate the inhibitory ability of tPA, active PAI-1 quickly loses its activity with time as a result of conformational changes. We therefore believe that the activity assay does not reflect the real PAI-1 activity of pla...

Rationale: The antiangiogenic activity of rPAI-123, a truncated plasminogen activator inhibitor-1 (PAI-1) protein, induces vasa vasorum collapse and significantly reduces plaque area and plaque cholesterol in hypercholesterolemic low-density lipoprotein receptor–deficient/apolipoprotein B48–deficient mice. Objective: The objective of this study was to examine rPAI-123–stimulated mechanisms that...

Bleeding diathesis is a common reason for investigation of the haemostatic system and in about 50% of the cases an abnormality is found. Plasminogen activator inhibitor type 1 (PAI-1) is an inhibitor of tissueand urokinase plasminogen activator (tPA/uPA). High PAI-1 levels are associated with arterial and venous thrombotic conditions but are also seen in pregnancy, inflammatory and infectious d...

PURPOSE To quantify the basal free t-PA activity in human aqueous humor. METHODS Human aqueous humor obtained by simplified pipette paracentesis at cataract surgery was tested for free t-PA activity in a revised 15-hour amidolytic assay using a t-PA standard curve (n = 15). Total antigenic levels of PAI-1, the principal PA inhibitor, were determined using an ELISA kit. The available PAI-1 act...