Different concentrations and combinations of hormones were used in MS medium to observe callus induction and then plant regeneration using leaf as explant. The rate of callus formation varied in different treatments used. The highest amount of callus (80.67%) was produced on MS medium containing 3.0 mg/l NAA from leaf after 20 days. The highest fresh weight of callus was obtained from 2.0 mg/l ...

Nilam Aceh ‘Sidikalang’ (Acehnese Patchouli) merupakan salah satu anggota kelompok dari Lamiaceae, famili mint. Minyak aromatik yang biasa disebut juga dengan minyak patchouli dapat diproduksi daunnya, mana bernilai tinggi dalam industri parfum dan aromaterapi, karena wangi segar. memiliki variasi senyawa fitokimia efek terapeutik, seperti antimikroba, antidepresan, anti-inflamasi, antioksidan....

A Keiki is the product of asexual propagation which naturally develop at node a flower stalk or cane. Phalaenopsis produce along middle to end nodes on stalk. Although keikis are an inefficient way propagate orchids commercial scale, it interesting study cytokinin hormone Benzyl Aminopurine (BAP) effect stimulate Two each were used in this experiment. Cytokinin (0 mg L −1 , 2 000 4 6 BAP) was o...

Populations of Agave potatorum Zucc. have been overexploited from their habitat for the commercial production mezcal, a traditional Mexican liquor. For this species, micropropagation is only method cloning selected genotypes. The aim study was to establish an efficient protocol in-vitro propagation A. by use individual shoots. In stage, interaction between different concentrations cytokinin ben...

An in vitro experiment was conducted to find out the optimum hormonal supplement and sucrose level for the bulb production of Hippeastrum. Murashige and Skoog medium supplemented with different hormone concentrations of BAP (0.0, 2.0, 4.0, 6.0 and 8.0 mg/L) and CCC (0.0, 125, 250 and 500 mg/L) and sucrose levels (30, 60, 80, 90 and 110 g/L) were used in this study. Sucrose level at 90 g/L produ...

An efficient protocol was developed for the regeneration of plantlets via direct and indirect organogenesis from stem nodal segments of Naravelia zeylanica (L.) DC. (Ranunculaceae). The interaction of 6-benzyl aminopurine (BAP) with -naphthalene acetic acid (NAA) in Murashige and Skoog (1962) medium induced a varied morphological response. A maximum of 17.6 ± 1.5 shoots per explant were obtaine...

The present study was conducted to investigate the effects of different concentrations and combinations of plant growth regulators (PGRs) on callus induction of Tecomella undulata (Sm) Seem. The leaf segments were used as explants and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins such as NAA, 2,4-D, IAA and cytokikinins like BAP and KIN alone a...