the present work was performed to show the possible interaction of gab a, and gabab receptors in catalepsy. the following results were obtained: 1- muscimol (mus), a gabaa agonist, induced catalepsy. this effect was dose-dependent. baclofen (bac), a gabab agonist, had no effect in this respect. 2- catalepsy induced by muscimol was potentiated by pretreatment of animals with bicuculline (bic), a...

In the postnatal subventricular zone (SVZ) local cues or signaling molecules released from neuroblasts limit the proliferation of glial fibrillary acidic protein (GFAP)-expressing progenitors thought to be stem cells. However, signals between SVZ cells have not been identified. We show that depolarizations of neuroblasts induce nonsynaptic SNARE-independent GABAA receptor currents in GFAP-expre...

In a previous report, we demonstrated that ginsenoside Rc, one of major ginsenosides from Panax ginseng, enhances γ-aminobutyric acid (GABA) receptorA (GABAA)-mediated ion channel currents. However, little is known about the effects of ginsenoside metabolites on GABAA receptor channel activity. The present study investigated the effects of ginsenoside metabolites on human recombinant GABAA rece...

Modulation of receptor trafficking is critical for controlling neurotransmission. A γ2(R43Q) point mutation on GABAA receptor subunit is linked to epilepsy in human. We recently analyzed the effect of this amino-acid substitution on GABAA receptor trafficking and showed that this mutation as well as agonist application, both affecting GABAA receptor extracellular domain, have an effect on recep...

Imidazo[1,5-a][1,4]benzodiazepines are well documented to exhibit potent activity at GABAA/Bz receptors. This series belongs to one of the very few chemical families, which have been extensively investigated for GABAA/Bz receptor mediated activity.1,2 Flumazenil (Ro 15-1788), an imidazo[1,5-a][1,4]benzodiazepine, was earlier shown to bind to central GABAA receptors with little or no intrinsic a...

Gustav Akk has a background in biophysics and pharmacology of ion channels. His current work focuses on the mechanisms of activation and modulation of transmitter-gated ion channels, particularly the GABAA receptor, under physiological and clinical conditions. The GABAA receptor is the major ionotropic inhibitory ion channel in the brain and an important component in the maintenance of excitato...

There is little doubt regarding the therapeutic possibilities of modulation of GABAA receptor function as exemplified by the clinical utility of benzodiazepines for half a century. The emerging understanding of the role of different GABAA receptor subtypes in mediating different physiological functions and pathological processes continues to offer opportunities for novel therapeutics. However, ...

Dohi, Akiko, ERK/MAPK Pathway Regulation of GABAA Receptor Function. Doctor of Philosophy (Pharmacology and Neuroscience), December, 2009, pp116, 23 illustrations, 6 supplemental illustrations, bibliography, 158 titles. GABAA receptor is a ligand-gated ion channel that conducts negatively charged chloride ions. Influx of this ion leads to hyperpolarization of neurons; thus, suppression of the n...

Polyclonal antibodies were raised to synthetic amino acid sequences of the bovine GABAA receptor qand as-subunits and purified by affinity chromatography on a column coupled with the respective peptide. Anti-peptide %(41&424) and anti-peptide aJ459-467) antibodies immunoprecipitated GABAA receptors and recognized a protein of 53 kDa (PJ and 59 kDa (P& respectively, in Western blots of GABAA rec...

objective(s):the mechanism of hypothermia action of acetaminophen (apap) remains unclear even 125 years after its synthesis. acetaminophen produces hypothermia. the mechanism of this reduction in core body temperature is not clear but evidence shows that it is not dependent on opioid and cannabinoid receptors. because of strong documents about the roles of gaba and benzodiazepine receptors in h...