Polycomb repressive complex 2 (PRC2) modifies chromatin to maintain genes in a repressed state during development. PRC2 is primarily associated with CpG islands at repressed genes and also possesses RNA binding activity. However, the RNAs that bind PRC2 in cells, the subunits that mediate these interactions, and the role of RNA in PRC2 recruitment to chromatin all remain unclear. By performing ...

S05-01 Polycomb repressive complexes are required to maintain compact chromatin structure at Hox loci Ragnhild Eskeland, Duncan Sproul, Graeme Grimes, Shelagh Boyle, Martin Lieb, Clemence Kress, Nick Gilbert, Helle F. Jorgensen, Amanda G. Fisher, Arthur I. Skoultchi, Anton Wutz, Wendy A. Bickmore 1 University of Edinburgh, Edinburgh, United Kingdom 2 Research Institute of Molecular Pathology, V...

S05-01 Polycomb repressive complexes are required to maintain compact chromatin structure at Hox loci Ragnhild Eskeland, Duncan Sproul, Graeme Grimes, Shelagh Boyle, Martin Lieb, Clemence Kress, Nick Gilbert, Helle F. Jorgensen, Amanda G. Fisher, Arthur I. Skoultchi, Anton Wutz, Wendy A. Bickmore 1 University of Edinburgh, Edinburgh, United Kingdom 2 Research Institute of Molecular Pathology, V...

X chromosome inactivation (XCI) achieves dosage balance in mammals by repressing one of two X chromosomes in females. During XCI, the long noncoding Xist RNA and Polycomb proteins spread along the inactive X (Xi) to initiate chromosome-wide silencing. Although inactivation is known to commence at the X-inactivation center (Xic), how it propagates remains unknown. Here, we examine allele-specifi...

Direct binding of WD40 repeat of Embryonic Ectoderm Development (EED) in the Polycomb Repressor Complex 2 (PRC2) to the histone H3 tail regulates the H3K27 methyltrasnferase activity of PRC2. The binding activity is required for the methylation of H3K27 over long distance of a Drosophila chromosome from the UBX (Hox gene) promoter to the PBX upstream enhancer region, implying EED initiates prop...

S05-01 Polycomb repressive complexes are required to maintain compact chromatin structure at Hox loci Ragnhild Eskeland, Duncan Sproul, Graeme Grimes, Shelagh Boyle, Martin Lieb, Clemence Kress, Nick Gilbert, Helle F. Jorgensen, Amanda G. Fisher, Arthur I. Skoultchi, Anton Wutz, Wendy A. Bickmore 1 University of Edinburgh, Edinburgh, United Kingdom 2 Research Institute of Molecular Pathology, V...

Transcription in the vicinity of DNA double-strand breaks (DSBs) is suppressed via a process involving ataxia telangiectasia mutated protein (ATM) and H2AK119 ubiquitylation.(1) We discuss recent findings that components of the Polybromo and Brahma-related gene 1 (BRG1)-associated factor (PBAF) remodeling complex and the polycomb repressive complex (PRC1/2) are also required.(2) Failure to acti...