Gene flow among crops and their wild relatives is an active study area in evolutionary biology horticulture, because genetic exchange between them may impact trajectories increase the variation of cultivated lineages. Mexico a center diversity for genus Cucurbita that includes pumpkins, squash gourds. domesticated species has been reported as common ; but gene populations C. pepo ssp. from its ...

Reanalysis and direct accelerator mass spectrometry radiocarbon dating of the cucurbit assemblage from Coxcatlan Cave provide information on the timing and sequence of the initial appearance of three domesticated plants in the Tehuacán Valley (Puebla, Mexico) and allow reassessment of the overall temporal context of plant domestication in Mexico. Cucurbita pepo is the earliest documented domest...

The gene coding for the 109-amino-acid, non-glycosylated form of mavicyanin was synthesized and expressed in Escherichia coli. The recombinant protein refolded from E. coli inclusion bodies was purified and characterized. Its spectroscopic properties are fully identical to those of mavicyanin isolated from zucchini, even in the absence of its carbohydrate moiety. The blue cooper center of mavic...

The study evaluated the quantity of Polyaromatic hydrocarbon (PAH) present in aqueous ethanol (Aq. EtOH), dichloromethane (DCM), and n-hexane (n-hex) extracts pumpkin (Cucurbita pepo) seed. Aliquots dried extracts, gotten from ground C. pepo, were subjected to Gas Chromatography Flame-Ionization Detector (GC-FID) analysis for quantitative identification polycyclic aromatic hydrocarbons (PAHs) s...

A gene encoding an endopeptidase from Streptococcus parasanguis FW213 has been cloned and shown to have high sequence homology to genes encoding mammalian metalloendopeptidases. The gene, designated S. parasanguis pepO, was cloned into the pET28a expression vector, resulting in a fusion of vector sequences encoding a hexahistidine tag at the carboxyl terminus. The recombinant PepO (rPepO) was e...