هدف: از چالش­های عمده بشریت افزایش افسردگی و اختلال عملکرد جنسی ناشی داروهای ضدافسردگی است. با توجه به نقش سلول­های سرتولی در اسپرماتوژنز، پژوهش حاضر، اثر داروی دولوکستین را بر زنده­مانی، آپوپتوزیس بیان ژن­های Bax و­ (Connexin 43) Cx43 بررسی کرده مواد روش‌‌ها: TM4 محیط DMEM/F12 حاوی %5/2 FBS، 5% سرم اسب %1 پنی سیلین-استرپتومایسین کشت شدند. دوزهای 30،60، 15، 5/7، 75/3 میکرو­گرم/ میلی­لیتر زمان­ه...

Samples of bulk tank milk from dairies across the United States, taken as part of the National Animal Health Monitoring Dairy 2002 survey, were analyzed for the presence of several genes encoding virulence factors associated with enterohemorrhagic forms of Escherichia coli (EHEC) using real-time and conventional PCR assays. Samples from 859 farms in 21 states were collected and enriched in EC m...

BACKGROUND & OBJECTIVES El Tor Vibrio cholerae O1 carrying ctxB C trait, so-called El Tor variant that causes more severe symptoms than the prototype El Tor strain, first detected in Bangladesh was later shown to have emerged in India in 1992. Subsequently, similar V. cholerae strains were isolated in other countries in Asia and Africa. Thus, it was of interest to investigate the characteristic...

Since enterohemorrhagic Escherichia coli (EHEC) isolates of serogroup O156 have been obtained from human diarrhea patients and asymptomatic carriers, we studied cattle as a potential reservoir for these bacteria. E. coli isolates serotyped by agglutination as O156:H25/H-/Hnt strains (n = 32) were isolated from three cattle farms during a period of 21 months and characterized by rapid microarray...

The incidence of Vibrio cholerae non-O1, non-O139 strains from hospitalized patients with acute diarrhea constituted 27.4% (n = 54) of the total 197 V. cholerae strains isolated from patients in Kolkata, India, in 2003. Of 197 strains, 135 were identified as O1 serotype Ogawa and 2 were identified as O139. In the same time period, six O1 background rough strains that possessed all known virulen...

Clostridium difficile causes pseudomembranous colitis through the action of Rho-modifying proteins, toxins A and B. Antibodies directed against C. difficile toxin A prevent or limit C. difficile-induced colitis. We engineered plasmid pETR14, containing the hlyB and hlyD genes of the Escherichia coli hemolysin operon, to express a fusion protein containing 720 amino acid residues from the nontox...