Aim. To compare changes in the complement system C3-glomerulopathy (C3-GP) and atypical hemolytic uremic syndrome (aHUS) after relief of an acute episode thrombotic microangiopathy.
 Materials methods. The study included 8 patients diagnosed with C3-GP aHUS remission. blood levels components were determined: C3, C4, C3a, C5a, factor H (CFH), B (CFB), membrane-attacking complex (MAC), antib...

Serum levels of complement components and of C3 nephritic factor (C3NeF) were measured serially in two patients with membrano-proliferative glomerulonephritis who were subjected to bilateral nephrectomy and maintained by peritoneal dialysis for 2 wk before renal transplantation. In both patients, low levels of C3 and high levels of preformed alpha 2D, a C3 breakdown product, were present before...

We present evidence suggesting that nonenzymatic, spontaneous hydrolysis of a thioester bond in native C3 constitutes the initial event in the activation of the alternative pathway. It has been known since the original description of C3 that the hemolytic activity of the isolated protein decays spontaneously in aqueous solution at a slow rate (1). This decay was greatly accelerated by chaotropi...

Herein we report directed iridium-catalyzed C3−H silylation of furfuryl imines, which grants access to versatile synthetic platforms. This transformation was developed on derivatives, using imines as directing groups, and trialkylsilanes or bis(trimethylsilyl)methylsilane silylating agents, in the presence a hydride scavenger. Subsequently, fluoride-mediated activation strategies were applied C...

We examined the interaction of complement component C3 with surface molecules on Trypanosoma cruzi. Five- to six-fold more C3 was bound to epimastigotes (Epi) than to metacyclic trypomastigotes (CMT) of strain M88. Epi and CMT were surface iodinated, then incubated in C8-deficient serum, and detergent lysates were applied to anti-C3 antibody that had been coupled to Sepharose. We found that 9.2...

Two complex enzymes were assembled that both converted C3 to C3b, one consisting of activated properdin (P), native C3, proactivator (PA) and proactivator convertase (PAase), and the other of nephritic factor (NF) and the same three cofactors. By maintaining a critical concentration of PAase, the P-C3 convertase and the NF-C3 convertase were shown to function efficiently without formation of th...

BACKGROUND Myocardial infarction (MI) is a major clinical problem because of its large contribution to mortality. The genetic bases of this disease have been widely studied in recent years to find a clear association with some genetic markers that increase the risk of its occurrence. In the present investigation, the correlation between MI and the C3 complement polymorphism was analyzed using a...

C3-bearing immune complexes and C3 activation products were detected by using two monoclonal antibodies, one specific for a neoantigenic determinant on C3c and the other for C3d. To quantitate immune complexes, the anti-C3c or anti-C3d antibodies were fixed to microtiter plates and reacted with test plasma. The binding of C3-bearing immune complexes in this plasma was then measured with radiois...