Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....

The Chinese giant salamander (Andrias davidianus) is the biggest extant and endangered amphibian, which has an incongruous sex ratio during artificial breeding process indistinguishable in appearance. development mechanism of this rare species thus needs to be explored. We therefore conducted study explore molecular mechanisms hallmarks or gonad maintenance genes for CGS. In study, we sequenced...

Sheep fertility genes have been studied in Turkey for many years. But so far it cannot be said that a satisfactory result has achieved terms of the number detected polymorphisms on genes. Exhibiting genetic structure local gene resources adapted to particular region is very important sustainability, conservation and breeding studies, other issues such as profitability. Therefore, primary aim th...

This research aimed to assess the genetic polymorphism of BMP15 gene concerning sex hormone levels and birth type in Awassi ewes. The genome DNA 138 ewes was isolated (52 produced a singleton 86 twins). exon-2 amplifiable by polymerase chain reaction (PCR), so two genotypes were identified based on 141 bp amplicons: TT TA. A sequencing revealed novel mutation, c.50980646T>A, TA genotype. single...

Background: This study was set up to evaluate the effect of ovarian tissue slow freezing on in vitro growth and pattern of maturation genes expression in mouse preantral follicles encapsulated within alginate hydrogel. Materials and Methods: Ovaries of 12-14 days old female NMRI mice were randomly allocated into control and slow freezing groups. In slow freezing group, ovaries were equilibrated...