The Hrp (type III protein secretion) system is essential for the plant parasitic ability of Pseudomonas syringae and most Gram-negative bacterial plant pathogens. AvrB and AvrPto are two P. syringae proteins that have biological activity when produced via heterologous gene expression inside plant cells or when produced by Hrp+ bacteria. Avr-like proteins, presumably injected by the Hrp system o...

hrp genes are reportedly required for pathogenicity in Pseudomonas syringae pv. syringae (Pss) and other phytopathogenic bacterial species. A subset of these genes encodes a type III secretion system through which virulence factors are thought to be delivered to plant cells. In this study, we sought to better understand the role that hrp genes play in interactions of Pss with its host as they o...

Este artículo presenta el enfoque de paridad riesgo jerárquico (HRP, por sus siglas en inglés) propuesto López Prado para la construcción porta­folios óptimos inversión empleando aprendizaje no supervisado –clustering jerárquico–, que permitan superar algunas limitaciones propias del modelo Media-Varianza, particular aquella relacionada con necesidad invertir matriz covarianzas momento implemen...

In this article, the authors present a conceptual framework named adaptive seriational risk parity (ASRP) to extend hierarchical parity (HRP) as an asset allocation heuristic. The first step of HRP (quasi-diagonalization), determining hierarchy assets, is required for actual done in second (recursive bisectioning). original scheme, found using single-linkage clustering correlation matrix...

Resonance Raman spectra of horseradish peroxidase (HRP) in the 200 to 600 cm" region were measured for the reduced and oxidized states and in the presence and absence of substrate, For the reconstituted HRP with a S4Fe-substituted heme, the Raman line of ferro HRP at 244 cm" was shifted to 246 cm" but all other modes remained unshifted. The amount of the frequency shift agreed with that observe...

Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles conta...

  Background and Objectives: Quality of work life is an increasingly important organizational factor in health facilities. Most studies on quality of work life in hospitals have been conducted in developed countries. The few studies performed in developing countries have targeted low- and middle-performance hospitals, and they have not explored the relationship between quality of work life and...

BACKGROUND The purpose of this study was to devise a novel electrochemical immunosensor for ultrasensitive detection of alfa-fetoprotein based on Fe(3)O(4)/Au nanoparticles as a carrier using a multienzyme amplification strategy. METHODS AND RESULTS Greatly enhanced sensitivity was achieved using bioconjugates containing horseradish peroxidase (HRP) and a secondary antibody (Ab(2)) linked to ...

The plant pathogenic bacterium Pseudomonas syringae is divided into pathovars differing in host specificity, with P. syringae pv. syringae (Psy) and P. syringae pv. tomato (Pto) representing particularly divergent pathovars. P. syringae hrp/hrc genes encode a type III protein secretion system that appears to translocate Avr and Hop effector proteins into plant cells. DNA sequence analysis of th...

To overcome the sensitivity limit for small molecules (haptens) in immunoassays based on antigen-horseradish peroxidase (HRP) conjugates as labels, a novel approach was established that afforded very low detection limits. Biotinylated anti-HRP antibody was utilized in order to attach, via a streptavidin bridge, liposomaly entrapped HRP. Fentanyl, used as a model antigen, could be determined via...