The importin/exportin transport system provides the machinery involved in nucleocytoplasmic transport. Alterations of the levels of importins and exportins may play crucial roles in development, differentiation and transformation. Employing human leukaemia HL-60 cells, we and others have revealed the differentiation-associated changes in the protein and gene expression of these factors. The rec...

Sesquiterpenes are a rich source of covalent inhibitors with long history in traditional medicine and include several important therapeutics tool compounds. Herein, we report the total synthesis 16 sesquiterpene lactones via build/couple/pair strategy, including goyasensolide. Using an alkyne-tagged cellular probe proteomics analysis, discovered that goyazensolide selectively targets oncoprotei...

sol and GTP hydrolysis. In particular, nuclear import Far from being passive participants in the process of is accomplished for proteins bearing classical nuclear mitosis, chromosomes have an active and essential role localization signals (NLS) by a heterodimeric receptor in the assembly of spindles required for their accurate comprised of a Ran-GTP binding protein, importin b, division into th...

The four serotypes of dengue virus (DENV-1 to -4) cause the most important arthropod-borne viral disease of humans. DENV non-structural protein 5 (NS5) contains enzymatic activities required for capping and replication of the viral RNA genome that occurs in the host cytoplasm. However, previous studies have shown that DENV-2 NS5 accumulates in the nucleus during infection. In this study, we exa...

The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. As PB2 is a relevant host-range determinant we expressed a TAP-tagged PB2 in human cells and isolated intracellular complexes. Alpha-importin was identified as a PB2-associated factor by proteomic analyses. To study the relevance of t...

Virtually all DNA viruses including hepatitis B viruses (HBV) replicate their genome inside the nucleus. In non-dividing cells, the genome has to pass through the nuclear pore complexes (NPCs) by the aid of nuclear transport receptors as e.g. importin beta (karyopherin). Most viruses release their genome in the cytoplasm or at the cytosolic face of the NPC, as the diameter of their capsids exce...