AIM To compare the sensitivity of doxorubicin (DOX) sensitive and DOX-resistant MC-rhabdomyosarcoma (MC-RMS) cells to the action of lymphokine-activated cells (LAC). RESULTS In vitro investigations showed that LAC received from the fraction of adherent lymphocytes possess the highest activity against DOX-resistant tumor cells, and LAC from lymphocytes of total pool--against DOX-resistant tumo...

In contrast to the ribosomal RNA gene expression increasing with growth rate, transcription of the lac operon is downregulated by cell growth rate. In continuous culture, growth rate regulation of lac promoter was independent of carbon substrate used and its location on the chromosome. Since the lac operon is activated by cyclic adenosine monophosphate (cAMP), which decreases with increasing ce...

Background: Cultural issues are important for diagnostic validity between different countries; little has been addressed from Latin America and Caribbean countries (LAC). Objective: To identify LAC studies on culture-bound syndromes (CBS) and extract potential empirical evidence about Susto, Nervios and Ataques de Nervios. Methods: Search strategies were carried out in Medline, Embase, Lilacs, ...

We present a new well defined sample of BL Lac objects selected from the ROSAT All-Sky Survey (RASS). The sample consists of 39 objects with 35 forming a flux limited sample down to fX(0.5− 2.0 keV) = 8 · 10 ergs cm s, redshifts are known for 33 objects (and 31 of the complete sample). X-ray spectral properties were determined for each object individually with the RASS data. The luminosity func...

Competition for foreign direct investment (FDI) among developing countries has intensified in recent years. Using a sample of 68 developing countries across dif ferent regions, with data from 1975-2005, this paper investigates whether Latin America and the Caribbean (LAC) dif fers from non-LAC regions in regard to determinants of FDI; the evidence suggests that there are dif ferences. In partic...

Escherichia coli lactose repressor (LacI), a tetrameric protein, is a paradigmatic transcriptional factor that controls the expression of lacZYA in the lac operon. It specifically binds to the O1, O2, and O3 operators of the lac promoter, forms DNA loops, and regulates transcription of the lac operon. In this article, utilizing combined techniques of DNA-nicking assay and AFM imaging, we direct...

Introduction of plasmids carrying the lacY gene (lactose permease gene) into Yersinia enterocolitica results in cells being able to ferment both lactose and raffinose. Transfer of such plasmids into Escherichia coli C600 (lacY) confers ability to ferment lactose but not raffinose. Derivatives of C600 that ferment both lactose and sucrose (Lac+ Scr+ strains) are able to ferment raffinose, but do...

A Lac+ mutant of Erwinia chrysanthemi was isolated from the Lac- wild type on lactose agar. beta-Galactosidase was expressed independently of lactose transport in both the mutant and the wild type, and neither strain expressed thiogalactoside transacetylase. Lactose transport and alpha-galactosidase, constitutive in the Lac+ strain, were coordinately induced in the Lac- strain by melibiose and ...

Chronic lymphocytic leukemia (CLL) is the most commonly observed adult hematological malignancy in Western countries. Despite the fact that recent improvements in CLL treatment have led to an increased percentage of complete remissions, CLL remains an incurable disease. Cerrena unicolor is a novel fungal source of highly active extracellular laccase (ex-LAC) that is currently used in industry. ...

The hydrophilic part of membrane proteins plays an important role in the formation of 3D crystals. The construction of fusion proteins using well crystallizing proteins as fusion partners is a possibility to increase the hydrophilic part of membrane proteins lacking large hydrophilic domains. These fusion proteins might be easier to crystallize. Two bifunctional fusion proteins containing the m...