Increasing Na delivery to epithelial Na channels (ENaC) in the connecting tubule (CNT) dilates the afferent arteriole (Af-Art), a process we call connecting tubule glomerular feedback (CTGF). We hypothesize that aldosterone sensitizes CTGF via a nongenomic mechanism that stimulates CNT ENaC via the aldosterone receptor GPR30. Rabbit Af-Arts and their adherent CNTs were microdissected and simult...

objective: alchornea floribunda leaves are widely used in ethnomedicine for the management of immuno-inflammatory disorders. we investigated the in vivo and in vitro antioxidant activity of the leaf extract, fractions and isolated compounds of a. floribunda.materials and methods: the ethyl acetate fraction of the methanol leaf extract was subjected to several chromatographic separations to isol...

The human P2Y1 sequence of 373 amino acids was terminated at position 354 (construct P2Y1-T1) or at position 334 (P2Y1-T2), covering almost all of the tail. These proteins were N-terminally tagged with the Myc epitope, giving Myc-P2Y1, Myc-P2Y1-T1 and MycP2Y1-T2, and expressed separately in 293 cells: reaction with fluorescent anti-Myc antibody showed in confocal microscopy that all three are t...

Isolates of Agaricus bisporus strains F56 and U3 were tested for sensitivity to several selected fungicides in vitro. The analysis showed that flusilasole + carbendazim and cyproconazole + carbendazim were the most toxic fungicides to A. bisporus strain F56 with respective EC50 values of 0.04 and 0.23 mg/l. The least toxic fungicides were carbendazim (EC50 = 16.58 mg/l) and trifloxystrobin (EC5...

The occurrence of antibiotic residues in the aquatic environment is an emerging concern. In contrast to daphnia and fish, algae are known to be particularly sensitive to antibiotic exposure. However, to date, a systematic evaluation of the sensitivity of different algal species to antibiotics has not been performed. The aim of the present study was therefore to explore the sensitivity of a batt...