نتایج جستجو برای: مارکر issr
تعداد نتایج: 3120 فیلتر نتایج به سال:
Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR ampli...
micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...
DNA markers help detect the intraspecific genetic diversity of yeast strains. Eight ISSR (Inter Simple Sequence Repeats) primers were used to assess Saccharomyces cerevisiae (n = 96) from different populations 3), evaluate technological characteristics, and investigate trait-loci associations. The amplified 154 reproducible scorable bands, which 79.87% polymorphic. UPGMA (unweighted pair group ...
مارکرهای dna از جمله مواد رایج در بیولوژی مولکولی می باشند.استانداردهای وزنی مولکولی dna ترکیبی از قطعات dna (دو رشته ای یا تک رشته ای) با اندازه شناخته شده می باشند. به صورت معمول، نمونه dna و یک مارکر در حفرات مجاور هم در ژل آگارز بارگذاری می شوند. dnaبه وسیله الکتروفورز در ژل جداشده و اندازه قطعات نمونه به وسیله مقایسه میزان حرکت با باندهای شناخته شده مارکر، معین می گردد. هدف از مطالعه حاضر ...
Fokienia hodginsii (Cupressaceae) is distributed in montane evergreen forests in North and Central Vietnam and extends to southeastern China at 900 m above sea level. The species has been threatened in its area of distribution in recent years because of habitat destruction and over-exploitation. The genetic variation of F. hodginsii in Vietnam was investigated on the basis of eight ISSR markers...
Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it large size has a yellow fruit skin color with greenish tips. However, problem production limited amount good quality seeds. The utilization vitro culture techniques on pineapples allows emergence somaclonal variations, especially plantlets that have undergone su...
The chemical composition of the essential oils of five natural populations of P. ovalifolia from central and southwest China and their interpopulation variability were first analyzed by using GC-MS. Twenty-two essential oil compounds were obtained, in which eighteen ones were identified and characterized representing 95%-96% of the oil composition. Three main chemotypes, i.e., the methyl-acetyl...
Information on the genetic identity and interrelationships of different Morus spp. genotypes is essential for their proper conservation and utilization. Hence, the present investigation was undertaken with genetically more reliable DNA markers to study the genetic relationship among eleven selected genotypes of mulberry collected from Japan, India and Italy. Using ten RAPD and ten ISSR primers,...
Plant molecular identity (ID) is used to describe molecular characteristics of plants, which should contain all of the necessary information. Using inter-simple sequence repeat (ISSR) primers, molecular ID can be described in a way that reflects the polymerase chain reaction (PCR) conditions, annealing temperature, and the bands obtained in PCR amplification. A new complete molecular ID system ...
The population structure of Phytophthora capsici among asexual and sexual progenies was analyzed using ISSR. Thirty one parent three populations were assayed for genetic diversity 5 ISSR primers DNA from 120 offspring P. amplified. In total, 71 reproducible fragments obtained, which 100% polymorphic, revealing high polymorphism the isolates. Among them, percentages progeny isolates 100.00 77.46...
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