Intestinal lipoproteins, especially triglyceride-rich chylomicrons, are a major driver of metabolism, inflammation, and cardiovascular diseases. However, isolating intestinal lipoproteins is very difficult in vivo because they first secreted from the small intestine into mesenteric lymphatics. Chylomicron-containing lymph then empties subclavian vein thoracic duct to deliver components meal hea...

Incubation of phosphatidylcholine vesicles with isolated high density lipoproteins (HDL) or with whole plasma results in transfer of phospholipid into HDL. To investigate the mechanisms of this process, small unilamellar vesicles of egg phosphatidylcholine were incubated with human HDL3 (d 1.125 to 1.210 g/ml) or with plasma and were subsequently analyzed by density gradient ultracentrifugation...

Characterization of the hyperlipoproteinemia induced by feeding high-cholesterol diets to hypothyroid dogs was undertaken in an attempt to identify a lipoprotein pattern or a specific lipoprotein responsible for the atherosclerosis associated with such hyperlipoproteinemia. Various degrees of hyperlipidemia and atherosclerosis were produced during the diet, which was imposed for 3 months to mor...

Chylomicrons and very low density lipoproteins (VLDL) are produced by the intestine and these nascent particles are thought to be similar to their counterparts in intestinal lymph. To study the relationship between these lipoproteins within the cell and those secreted into the lamina propria and lymph, we have isolated enterocytes, lamina propria, and mesenteric lymph from rats while fasted and...

Dyslipidemia has been associated with premature atherosclerosis and other pathological conditions, and the determination of serum lipids is of great importance in health and disease. Most of the lipids are transported in plasma in the form of lipoproteins. Four classes of lipoproteins have been recognized by electrophoresis: chylomicrons, beta-, pre-beta-, and alpha-lipoproteins. Fredrickson, L...

THE FOLLOWING HUMAN LOW DENSITY LIPOPROTEINS WERE PREPARED: beta-lipoproteins of densities greater than 1.040 (A, B,C) a beta-lipoprotein of -S(1.063) = 5 (D), a lipoprotein of -S(1.063) = 19 (E), and a lipoprotein of -S(1.063) = 70 (F). Data are presented which show the immunochemical homogeneity of the D lipoprotein rabbit-anti-D lipoprotein system. Cross-reactions between antibody to A and D...

The pathogenesis and progression of atherosclerosis are integrally connected to the concentration and function of lipoproteins in various classes. This review examines existing and emerging approaches to modify low-density lipoprotein and lipoprotein (a), triglyceride-rich lipoproteins, and high-density lipoproteins, emphasizing approaches that have progressed to clinical evaluation. Targeting ...

Carbon tetralchloride was injected into normnal rabbits and the levels of Sf 3-12, 12-20, alnd(20-40 lasses of lipoproteins were determined during the course of these injections. A marked increase in concentration of these three classes of lipoproteins occurred during the administration of carl)on tetra(hloride. After the cessation of this drug, the lipoproteins of largest Sf rate returned to n...

Among the apoA-I-containing lipoproteins isolated by selected-affinity immunosorption from human serum and plasma, we have identified a subpopulation which, unlike the bulk of high density lipoproteins, has pre-beta electrophoretic mobility. This pre-beta subpopulation can be observed directly in fresh plasma by immunoelectrophoresis. It contains phospholipid and free and esterified cholesterol...