The influenza virus PB1-F2 is an 87-amino acid mitochondrial protein that previously has been shown to induce cell death, although the mechanism of apoptosis induction has remained unclear. In the process of characterizing its mechanism of action we found that the viral PB1-F2 protein sensitizes cells to apoptotic stimuli such as tumor necrosis factor alpha, as demonstrated by increased cleavag...

Identification curves were estimated for the English consonants /b,d,g/ using five-formant CV synthetic stimuli comprehensively sampling the F2 onset-F2 vowel acoustic space in the vicinity of /b,d,g/ locus equations [H. Sussman et al., J. Acoust. Soc. Am. 90, 1309-1325 (1991)]. The stimuli included 10 English monophthongal vowel contexts, 11 levels of F2 onset per vowel, and 3 levels of F3 ons...

BACKGROUND The fungus of Ganoderma is a traditional medicine in Asia with a variety of pharmacological functions including anti-cancer activities. We have purified an extracellular heteropolysaccharide fraction, PS-F2, from the submerged mycelia culture of G. formosanum and shown that PS-F2 exhibits immunostimulatory activities. In this study, we investigated the molecular mechanisms of immunos...

The peptides produced enzymatically from various plants have shown various biological activities including cytotoxicity. Different types of cytotoxic peptides have been reported from the seeds and leaves of Violaceae, Rubiaceae and Annonaceae families. In this study, we report purification and characterization of peptide(s) showing cytotoxic activity against A549 and HeLa cancer cell lines from...

N-n-butyl haloperidol iodide (F2), a novel compound, has shown palliative effects in myocardial ischemia/reperfusion (I/R) injury. In this study, we investigated the effects of F2 on the extracellular signal-regulated kinase kinase (MEK)/extracellular signal-regulated kinase (ERK)/Na(+)/H(+) exchanger (NHE)/Na(+)/Ca(2+) exchanger (NCX) signal-transduction pathway involved in H2O2-induced Ca(2+)...

Highly pathogenic avian influenza A viruses (HPAIV) of the H5N1 subtype occasionally transmit from birds to humans and can cause severe systemic infections in both hosts. PB1-F2 is an alternative translation product of the viral PB1 segment that was initially characterized as a pro-apoptotic mitochondrial viral pathogenicity factor. A full-length PB1-F2 has been present in all human influenza p...

OBJECTIVE Maternal undernutrition during gestation is associated with increased metabolic and cardiovascular disease in the offspring. We investigated whether these effects may persist in subsequent generations. DESIGN Historical cohort study. SETTING Interview during a clinic or home visit or by telephone. POPULATION Men and women born in the Wilhelmina Gasthuis in Amsterdam between Nove...

Integrins are cell surface receptors crucial for cell migration and adhesion. They are activated by interactions of the talin head domain with the membrane surface and the integrin β cytoplasmic tail. Here, we use coarse-grained molecular dynamic simulations and nuclear magnetic resonance spectroscopy to elucidate the membrane-binding surfaces of the talin head (F2-F3) domain. In particular, we...

Carter & Local [3] report acoustic data showing that in two varieties of British English, the liquids /l/ and /r/ are polarised in terms of ‘darkness’ along the F2 dimension; Leeds English has dark onset /l/ (low F2) and a light onset /r/ (high F2), and Newcastle English has the opposite pattern. Here we report data on liquid polarisation for Australian English, a variety reported to show a ver...

rH..il:ribllltlton.-tr'ee two-sample test is proposed tha t is an extension of the Wilcoxon test with arbitrary censoring on the right. The test is conditional on the pattern ~ha.t~TV:&l>ll~l..lll. The null hypothesis is H0 : F1(t) = F2(t) (t ~ T) agai nst either H1 : F1(t) < F 2(t) (t ~ T) or H2 : F1(t) < F2(t) or F1(t) > F2 (t) (t ~ T), '~here .l\,F2 are cumulative distributions (discrete or ...