In the search of facile and efficient methods for cellular delivery of peptide nucleic acids (PNA), we have synthesized PNAs conjugated to oligophosphonates via phosphonate glutamine and bis-phosphonate lysine amino acid derivatives thereby introducing up to twelve phosphonate moieties into a PNA oligomer. This modification of the PNA does not interfere with the nucleic acid target binding affi...

A fully automated solid-phase synthetic procedure for incorporation of positively charged guanidinium linkages into otherwise neutral PNA sequences has been employed. These DNG/PNA chimeras form [(DNG/ PNA)2‚DNA] triplexes upon binding to single strand or duplex DNA (with accompanying D-loop for the latter). The [(DNG/PNA)2‚DNA] triplexes of DNG/PNA T10, with DNA dA10, are more stable than DNA‚...

the southern shores of caspian sea is subjected to several atmospheric factors due to its geographical position. north atlantic oscillation(nao) one of the effective phenomenon's affects the most part of hemisphere included caspian sea. in this research negative and positive phases of nao considered with variability of temperature and precipitation of southern shores of caspian sea in a 30...

This paper introduces a novel silicon-on-insulator (SOI) metal–semiconductor field-effect transistor (MESFET) with an implanted N layer (INL-SOI MESFET) to improve the DC and radio frequency characteristics. The DC and radio frequency characteristics of the proposed structure are analyzed by the 2-D ATLAS simulator and compared with a conventional SOI MESFET (C-SOI MESFET). The simulated result...

Peptide nucleic acids (PNAs) have been developed for applications in biotechnology and therapeutics. There is great potential in the development of chemically modified PNAs or other triplex-forming ligands that selectively bind to RNA duplexes, but not single-stranded regions, at near-physiological conditions. Here, we report on a convenient synthesis route to a modified PNA monomer, thio-pseud...

introduction: the cell surface glycoconjugate and extracellular matrix may mediate extracellular signals which decide the fate of the cells during tissue interactions. the proper development of cornea needs cellular interaction between surface epithelium, neural crest and developing lens. materials and methods: 50 pregnant rats were chosen and the embryos from pernatal day11-to 20 and postnatal...

Peptide nucleic acid (PNA), a DNA synthetic analog, assembles into helical duplexes as a consequence of Watson-Crick hydrogen bonds formed between nucleobases but has an achiral pseudo-peptide backbone, in contrast to DNA which has a phosphodiester backbone. Placing ligandmodified monomers in complementary positions in PNA oligomers makes possible metal ion incorporation within the PNA duplex (...

The potential use of peptide nucleic acid (PNA) as a sequence-specific inhibitor of RNA translation is investigated in this report. Three different regions of the PML/RARalpha oncogene, including two AUG potential start codons, were studied as targets of translation inhibition by antisense PNA in a cell-free system. A PNA targeted to the second AUG start codon, which was shown previously to be ...