Background — Presence of microorganisms in sterile body sites leads to life-threatening infections. For early and accurate diagnosis of those infections, cultures of the sterile fluids have to be done. These cultures can not always detect the causative agents due to insufficent number or fastidious growing of the probable microorganisms in the material. Objectives — In this study, it was aimed ...

We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC) from positive BACTEC 12B broth cultures. A total of 107 sputum samples were processed and inoculated into Ogawa slants and BACTEC 12B vials. At a growth index (GI) > or=30, 1.0 ml of the 12B broth was removed, stored, and assayed with PCR. Molecular results were compared to those obtained by phenotypic...

A variety of blood culture media were compared to determine the optimal method for the isolation of Mycobacterium avium-intracellulare (MAI) from the blood of patients with AIDS. Simulated laboratory blood cultures and clinical blood cultures were tested. Glucose broth, Bactec aerobic medium, Kirschner's medium, and Bactec Middlebrook medium supported the growth of MAI. The Isolator system, a l...

The BACTEC NR730 blood culture system is a commercially available system which utilizes infrared spectrophotometry to detect bacterial growth in broth media inoculated with the blood specimen. The objective of this study is to compare this system with the conventional system used in our laboratory. A total of 479 blood samples were collected from adult patients in the Klang TAR Hospital and ino...

Background: Patients with fever of unknown origin (FUO) are elusive and challenging clinical cases. Timely detection and identification of blood borne pathogen is one of the most important functions of microbiology laboratory. Objectives: To evaluate the efficacy of BACTEC 9050 in comparison to conventional blood culture for detection of bacterial isolates in clinically suspected cases of fever...

The recently developed ESP Culture System II (AccuMed, Chicago, Ill.) was compared with radiometric BACTEC 460TB (Becton Dickinson, Towson, Md.) and with Lowenstein-Jensen medium for recovery of mycobacteria from over 2,500 clinical specimens both of respiratory and nonrespiratory origin, including blood. The majority of the 219 mycobacterial isolates (129) belonged to the Mycobacterium tubercu...

OBJECTIVE To evaluate available evidence on the diagnostic value of radioactivity-free cultivation and detection technologies for rapid detection of rifampicin resistance in Mycobacterium tuberculosis. METHODS A fully recursive literature search was conducted in PubMed, EMBASE, Biosis, Web of Science (all 1990-2010), CBMWeb (1978-2010), and Google Scholar. QUADAS items were used to evaluate t...

BACKGROUND Infectious diseases are a leading cause of morbidity and mortality in developing countries. The aim of this study was to compare the results of blood culture employing the conventional and BACTEC methods for detection of bacterial infection in Taleghani Hospital, Tehran. MATERIALS AND METHODS This was a descriptive study carried out for 3 months (March 2014-May 2014) on 272 inpatie...

A total of 463 respiratory specimens, all smear positive for acid-fast bacteria, were inoculated onto routine solid media and into BACTEC 7H12 Middlebrook medium for detection of mycobacterial growth. Conventional drug susceptibility testing (1% proportion method) was performed on Middlebrook 7H10/7H11 medium, and radiometric susceptibility testing was performed on BACTEC 7H12 medium. The avera...

The BACTEC 16B resin medium was developed to detect bacteremia in patients receiving antimicrobial therapy. Over a 9-month period, we compared the efficacy of the BACTEC 16B bottle to the conventional aerobic BACTEC 6B bottle. Of a total of 1,524 sets of blood cultures, 79 yielded presumed pathogens. Of these 79 sets, 42 (53.2%) were positive in both the 16B and 6B bottles, 23 (29.1%) were posi...