Purpose TO examine the ability of retinal pigment epithelial (RPE) cells to superantigen to T lymphoc e&t2 resent bacterial es. Methods RPE cells, er interferon gamma (INFg) treated or untreated, were coincubated with Jurkat T-cells, staphylococcal enterotoxin E (SEE), and PMA for 24 hours. Supernatants were collected for interleukin-2 (IL-2) assay. Raji B-calls were assayed for comparison. Bin...

PURPOSE This investigation examined concurrent validity of the Children's OMNI-Resistance Exercise Scale (OMNI-RES) of perceived exertion for 10- to 14-yr-old females (N = 25) and males (N = 25) performing unilateral biceps curl (BC) and knee extension (KE) isotonic exercises. METHODS The criterion variable was total weight lifted (Wt(tot)), determined separately for females and males during ...

OBJECTIVE To differentiate the human embryonic stem cells (hESCs) into the retinal pigment epithelium (RPE) in the defined culture condition and determine its therapeutic potential for the treatment of retinal degenerative diseases. METHODS The embryoid bodies were formed from hESCs and attached on the matrigel coated culture dishes. The neural structures consisting neural precursors were sel...

Age-related macular degeneration (AMD), the leading cause of blindness in the elderly, targets the retinal pigment epithelium (RPE), a monolayer of cells at the back of the eye. As AMD progresses, it can develop into two distinct forms of late AMD: "dry," atrophic AMD, characterized by RPE senescence and geographic RPE loss, and "wet," neovascular AMD, characterized by RPE activation with abnor...

Orthodenticle homeobox 2 (OTX2) controls essential, homeostatic retinal pigment epithelial (RPE) genes in the adult. Using cocultures of human CD14+ blood monocytes (Mos) and primary porcine RPE cells and a fully humanized system using human-induced pluripotent stem cell-derived RPE cells, we show that activated Mos markedly inhibit RPEOTX2 expression and resist elimination in contact with the ...

Human retinal pigment epithelium (RPE) contains two genetically distinct forms of superoxide dismutase (SOD) enzymes that scavenge harmful superoxide anions. Biochemical and immunochemical techniques were used to compare levels of copper-zinc- and manganese-containing forms of SOD (CuZn-SOD and Mn-SOD) in human adult and fetal RPE cells. It was found that Mn-SOD activity was higher in adult tha...

The purpose of this study was to compare the influence of measuring the overall session rating of perceived exertion (session-RPE) at 10 vs. 30 minutes following exercise. Eight boxers completed three different standardized training sessions of different intensities (easy, moderate and hard) in a matchedpairs, randomized research design. Exercise intensity was assessed during each bout by measu...

PURPOSE To demonstrate the production of hemoglobin by human retinal pigment epithelium (RPE). METHODS Proteomic analysis using 10 donor eyes identified hemoglobin as a major constituent of soluble human RPE proteome. Western blot analysis, RT-PCR, and immunocytochemistry were used to confirm the RESULTS The presence of erythrocyte-specific proteins within primary human RPE cytosol was inve...

Retinal pigment epithelial (RPE) cells are central to retinal health and homoeostasis. Dysfunction or death of RPE cells underlies many age-related retinal degenerative disorders particularly age-related macular degeneration. During aging RPE cells decline in number, suggesting an age-dependent cell loss. RPE cells are considered to be postmitotic, and how they repair damage during aging remain...

PURPOSE To evaluate the effectiveness of session rating of perceived exertion (RPE) to measure effort during different types of resistance training. METHOD Fifteen male subjects (age 26.7 +/- 4.3 years) performed 3 protocols. All protocols consisted of same 5 exercises but with different intensities, rest periods, and numbers of repetitions. One-repetition maximum (1-RM) was defined as the ma...