نتایج جستجو برای: روش lsu
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Dorsal-spined protostrongylid nematode larvae (Metastrongyloidea: Protostrongylidae) were recovered from the feces of the endangered pampas deer (Ozotocerus bezoarticus celer) in Campos del Tuyú Wildlife Reserve, Bahia Samborombón, Argentina. Partial DNA sequences from the large subunit ribosomal RNA (LSU rRNA) gene and from the second internal transcribed spacer region (ITS2) were amplified, c...
Phylogenetic analyses of 27 artillery fungus (Sphaerobolus sp.) isolates were conducted to identify species boundaries in the genus Sphaerobolus. Multiple gene genealogies inferred from maximum likelihood, Bayesian, and maximum-parsimony analyses of sequence data from individual loci (mtSSU, ITS, EF 1-alpha, and LSU) and a combined dataset (mtSSU, ITS, and EF 1-alpha) concordantly indicate the ...
هدف: هدف این پژوهش مقایسه اثربخشی امیددرمانی و معنویت درمانی بر کیفیت زندگی، قند خون فشار زنان میانسال مبتلا به دیابت نوع دو بود. روش: حاضر نیمه آزمایشی با طرح پیش آزمون- پس پیگیری گروه گواه جامعه آماری شامل تمام 2 مراجعه کننده مرکز تحقیقات دیابت، متابولیسم غدد شهر تهران در سال 1399 بودند که 45 نفر شیوه نمونهگیری دسترس انتخاب صورت تصادفی آزمایش یک (هر 15 نفر) قرار گرفتند. ابزار پرسشنامه کوتاه...
Moldy Boletus sp. and Xerocomus sp. were collected from several locations at the campus of the University of Tabriz, Iran. Fungicolous fungal isolates were recovered and characterized by the combination of morphological traits and phylogenetic analyses of combined ITS and LSU sequence data. Fungal isolates were identified as Sepedonium microspermum. This is the first ...
Four yeast strains were isolated from soil in a mixed deciduous forest in Amphoe Wang Nam Khiao, Nakhon Ratchasima province, Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analyses of the D1/D2 domain of the large-subunit (LSU) rRNA gene, small-subunit rRNA gene and internal transcribed spacer (ITS) region, the three stra...
Specimens of Dictyocaulus spp. were extracted from the respiratory tracts of 3 ruminant hosts including roe deer (Capreolus capreolus), red deer (Cervus elaphus), and chamois (Rupicapra rupicapra) from wild populations in the province of Le6n, northwestern Spain. The near-complete nuclear small-subunit ribosomal RNA gene, and 2 regions of the large-subunit ribosomal RNA gene, were amplified by ...
Rickettsia felis, the etiologic agent of spotted fever, is maintained in cat fleas by vertical transmission and resembles other tick-borne spotted fever group rickettsiae. In the present study, we utilized an Ixodes scapularis-derived tick cell line, ISE6, to achieve isolation and propagation of R. felis. A cytopathic effect of increased vacuolization was commonly observed in R. felis-infected ...
Predicting the secondary structure of RNA molecules from the knowledge of the primary structure (the sequence of bases) is still a challenging task. There are algorithms that provide good results e.g. based on the search for an energetic optimal configuration. However the output of such algorithms does not always give the real folding of the molecule and therefore a feature to judge the reliabi...
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