We have investigated the role of cytoplasmic dynein in microtubule organizing center (MTOC) positioning using RNA-mediated interference (RNAi) in Caenorhabditis elegans to deplete the product of the dynein heavy chain gene dhc-1. Analysis with time-lapse differential interference contrast microscopy and indirect immunofluorescence revealed that pronuclear migration and centrosome separation fai...

The Smith-Lemli-Opitz syndrome (SLOS) is an often lethal birth defect resulting from mutations in the gene responsible for the synthesis of the enzyme 3beta-hydroxy-steroid-Delta7-reductase, which catalyzes the reduction of the double bond at carbon 7 on 7-dehydrocholesterol (7-DHC) to form unesterified cholesterol. We hypothesize that the deficiency in cholesterol biosynthesis and subsequent a...

OBJECTIVE To determine the need for orthodontic treatment among Tanzanian Bantu children. DESIGN Prospective study using dental casts. SETTING Sixteen randomly selected schools from the Ilala district, Dar-es-Salaam, Tanzania. SUBJECT Six hundred and forty three Tanzanian children from an urban district. METHOD Dental casts of 643 Tanzanian subjects in age groups 3-5, 6-8, 9-11 and 15-1...

Delayed hydride cracking (DHC) remains an important phenomenon when considering cladding mechanical integrity during transport and storage of spent nuclear fuel. This work studies the hydrogen diffusion precipitation patterns DHC as a function temperature via high-resolution neutron imaging. Zircaloy-2 tubes with without inner liner were radially cracked from outside-in direction within range 2...

The paper presents the results of in situ studies marine particles different nature using a submersible digital holographic camera (DHC) during Arctic expedition. It also describes features, performance specifications, and possibilities DHC technology. technology can be used for noninvasive automatic evaluation spatial temporal characteristics plankton, including distribution plankton concentra...

Urban district heating and cooling (DHC) systems operate large freezers, heat exchangers, and boilers to stably and economically supply hot and cold water, steam etc., based on customers demand. We formulate an operation-planning problem as a nonlinear integer programming problem for an actual DHC plant. To reflect actual decision making appropriately, we incorporate contract-violation penaltie...

Abnormal axonal transport is associated with neuronal disease. We identified a role for DHC-1, the C. elegans dynein heavy chain, in maintaining neuronal cargo distribution. Surprisingly, this does not involve dynein's role as a retrograde motor in cargo transport, hinging instead on its ability to inhibit microtubule (MT) dynamics. Neuronal MTs are highly static, yet the mechanisms and functio...