1. A reaction center from chloroplasts was purified by means of detergent treatment, differential centrifugation, column chromatography, and sucrose gradient. 2. The reaction center is active in NADP photoreduction by ascorbate. Ferredoxin, ferredoxin-NADP-reductase, and plastocyanin were required for the reaction. 3. The preparation contains five classes of polypeptide chains with apparent mol...

Little is known about the role of mitochondrial NADP(+)-isocitrate dehydrogenase (NADP(+)-ICDH) in the heart, where this enzyme shows its highest expression and activity. We tested the hypothesis that in the heart, NADP(+)-ICDH operates in the reverse direction of the citric acid cycle (CAC) and thereby may contribute to the fine regulation of CAC activity (Sazanov and Jackson, FEBS Lett 344: 1...

In illuminated chloroplasts, one mechanism involved in reduction-oxidation (redox) homeostasis is the malate-oxaloacetate (OAA) shuttle. Excess electrons from photosynthetic electron transport in the form of nicotinamide adenine dinucleotide phosphate, reduced are used by NADP-dependent malate dehydrogenase (MDH) to reduce OAA to malate, thus regenerating the electron acceptor NADP. NADP-MDH is...

An NADPH-dependent NO2--reducing system was reconstituted in vitro using ferredoxin (Fd) NADP+ oxidoreductase (FNR), Fd, and nitrite reductase (NiR) from the green alga Chlamydomonas reinhardtii. NO2- reduction was dependent on all protein components and was operated under either aerobic or anaerobic conditions. NO2- reduction by this in vitro pathway was inhibited up to 63% by 1 mm NADP+. NADP...

When the extracellular concentration of glucose was raised from 3 mm to 7 mm (the concentration interval in which fl-cell depolarization and the major decrease in K+ permeability occur), the cytosolic free [NADPH]/ [NADP+] ratio in mouse pancreatic islets increased by 29.5% . When glucose was increased to 20 mm, a 117% increase was observed. Glucose had no effect on the cytosolic free [NADH]/[N...

1. The stoicheiometries and affinities of ligand binding to isocitrate dehydrogenase were studied at pH 7.0, mainly by measuring changes in NADPH and protein fluorescence. 2. The affinity of the enzyme for NADPH is about 100-fold greater than it is for NADP+ in various buffer/salt solutions, and the affinities for both coenzymes are decreased by Mg2+, phosphate and increase in ionic strength. 3...

The Baeyer-Villiger monooxygenases (BVMOs) are a family of bacterial flavoproteins that catalyze the synthetically useful Baeyer-Villiger oxidation reaction. This involves the conversion of ketones into esters or cyclic ketones into lactones by introducing an oxygen atom adjacent to the carbonyl group. The BVMOs offer exquisite regio- and enantiospecificity while acting on a wide range of subst...

Cytoplasmic NADP(+)-isocitrate dehydrogenase (NADP(+)-IDH) has been purified and characterized, and its gene sequenced in many animal, plant, and yeast species. However, much less information is available on this enzyme-gene in insects. As a first step in investigating the biochemical and molecular mechanisms by which NADP(+)-IDH contributes to adaptations for flight vs. reproduction in insects...

1. Glucose 6-phosphate dehydrogenase was isolated and partially purified from a thermophilic fungus, Penicillium duponti, and a mesophilic fungus, Penicillium notatum. 2. The molecular weight of the P. duponti enzyme was found to be 120000+/-10000 by gelfiltration and sucrose-density-gradient-centrifugation techniques. No NADP(+)- or glucose 6-phosphate-induced change in molecular weight could ...

Long-term and short-term effects of phytochrome on the levels (“tissue contents”) of NAD, N A D H ,, NADP and NADPH, were measured in the cotyledons of the mustard (Sinapis alba L.) seedling. It was found that long-term far-red light (which is considered to operate exclusively via active phytochrome, Pfr) strongly increases the levels of NADP and NADPH2 , whereas this light treatment suppresses...