Micromachining allows the formation of micrometer-sized regions of bare gold on the surface of a gold film supporting a self-assembled monolayer (SAM) of alkanethiolate. A second SAM forms on the micromachined surfaces when the entire system-the remaining undisturbed gold-supported SAM and the micromachined features of bare gold-is exposed to a solution of dialkyl disulfide. By preparing an ini...

Background: The relative changes in plasma and intracellular concentrations of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) may be important predictors of cellular methylation potential and metabolic alterations associated with specific genetic polymorphisms and/or nutritional deficiencies. Because these metabolites are present in nanomolar concentrations in plasma, methods of de...

The focus of analyzing data from microarray experiments has shifted from the identification of associated individual genes to that of associated biological pathways or gene sets. In bioinformatics, a feature selection algorithm is usually used to cope with the high dimensionality of microarray data. In addition to those algorithms that use the biological information contained within a gene set ...

Production of an experimental preparation of systolic anterior motion (SAM) of the mitral valve was attempted for the purpose of clarifying its causal factors. Fourteen thoracotomized dogs under pentobarbital anesthesia were administered dobutamine, and the echocardiogram, left ventricular pressure, and aortic pressure of each were simultaneously recorded. In two of the dogs in which SAM was pr...

Asymmetric septal hypertrophy (ASH) is considered the unifying link in the spectrum of hypertrophic cardiomyopathies. ASH and mitral valve systolic anterior motion (SAM) are the two most important echocardiographic criteria for the diagnosis of idiopathic hypertrophic subaortic stenosis (IHSS). Ten patients found have SAM without ASH were studied. Septal thickness, thickening, and excursion wer...

BACKGROUND We have developed an assay that uses stable-isotope-dilution liquid chromatography-mass spectrometry to assess S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in body fluids to investigate the relationship of these metabolites to hyperhomocysteinemia. METHODS Commercially obtained SAM (D(3) methyl) and (13)C(5)-SAH uniformly labeled in the adenosyl moiety, which was syn...

All above-ground plant organs are derived from shoot apical meristems (SAMs). Global analyses of gene expression were conducted on maize (Zea mays L.) SAMs to identify genes preferentially expressed in the SAM. The SAMs were collected from 14-day-old B73 seedlings via laser capture microdissection (LCM). The RNA samples extracted from LCM-collected SAMs and from seedlings were hybridized to mic...

The diverse reactions catalyzed by the radical-SAM superfamily of enzymes are thought to proceed via a set of common mechanistic steps, key among which is the reductive cleavage of S-adenosyl-L-methionine (SAM) by a reduced [4Fe-4S] cluster to generate an intermediate deoxyadenosyl radical. A number of spectroscopic studies have provided evidence that SAM interacts directly with the [4Fe-4S] cl...

Sam was diagnosed with extended oligoarticular Juvenile Idiopathic Arthritis at the age of 18 months. He began Etanercept at the age of 10 years old in March 2007 after an inadequate response to non-steroidal medications, methotrexate and numerous steroid preparations. Seventeen months later he was switched to Adalimumab with the expectation that this would have an improved response for him. Sa...