Successful targeting methods represent a major hurdle to the use of retroviral vectors in cell-specific gene-delivery applications. We recently described an approach for retroviral targeting with a retroviral receptor-ligand bridge protein that was bound to the cognate cell-surface ligand receptors before viral challenge. We now report a significant improvement made to this viral targeting meth...

The transcription factor Blimp-1 has emerged as a regulator of cell fate in embryonic (germ cell) and adult (B- and T-cell immune effector and epithelial) lineages. It has also been proposed to act as a tumor suppressor in B-cell malignancy. Here, we present a novel in vivo system enabling the targeted genetic manipulation of cells expressing Prdm1, the gene encoding Blimp-1. We created bacteri...

This is the second paper in a series in which we construct and analyze a class of TVB (total variation bounded) discontinuous Galerkin finite element methods for solving conservation laws ut + Ed I(fi(u))xi = 0. In this paper we present a general framework of the methods, up to any order of formal accuracy, using scalar one-dimensional initial value and initial-boundary problems as models. In t...

The determination of total volatile bases (TVB) is probably the oldest objective chemical method for assessing the spoilage of fishery pro­ ducts. It was proposed about a century ago and was already published as standard method for the inspection of fish in Germany in the first years of this century (KÖNIG, 1910). This simple method is still widely used. A European Union directive on fish hygie...

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Different kinds of lipid damage indices (peroxide value, conjugated diene index, thiobarbituric acid index, free fatty acids content, polyene index and fluorescent compounds formation) were studied during the chilled storage (0oC) of a lean fish species (blue whiting, Micromesistius poutassou) and compared to total volatile base-nitrogen content (...

We have seen important strides in our understanding of mechanisms underlying stroke recovery, yet effective translational links between basic and applied sciences, as well as from big data to individualized therapies, are needed to truly develop a cure for stroke. We present such an approach using The Virtual Brain (TVB), a neuroinformatics platform that uses empirical neuroimaging data to crea...