A new procedure is described for the reassociation of histone H1 to rat liver polynucleosomes selectively depleted of H1 and nonhistone proteins. The fidelity of reconstitution was scrutinized by nuclease digestion and sedimentation studies monitoring the regeneration of structural features which disappear following removal of H1. The results demonstrate that the 166-base pair barrier of digest...

Keratinocytes (KCs) may play important roles for maintenance of peripheral tolerance in the upper layers of the skin. Coinhibitory signals mediated via the programmed death (PD)-1 and its ligand B7-H1 (PD-L1/CD274) are crucial for the downregulation of T cell immune responses and for the maintenance of peripheral tolerance. In this study, to investigate the role of KC-expressed B7-H1 in the reg...

The structural requirements for DNA/RNA hybrids to be suitable substrates for RNase H1 are well described; however the tolerance level of this enzyme towards modifications that do not alter the duplex conformation is not clearly understood, especially with respect to the sense RNA strand. In order to investigate the molecular requirements of Escherichia coli RNase H1 (termed RNase H1 here) with...

BACKGROUND The discovery that genetic alterations in oncogenes and tumour suppressor genes accompany tumour formation in many human tumours has encouraged the search for genes that promote or suppress tumour spread and metastasis; nm23 is a promising candidate for a metastasis suppressing gene. AIMS To evaluate whether expression of nm23-H1 protein or loss of heterozygosity (LOH) of the nm23-...

Neuronal stimulation leads to immediate early gene (IEG) expression through calcium-dependent mechanisms. In recent years, considerable attention has been devoted to the transcriptional responses after neuronal stimulation, but relatively little is known about the changes in chromatin dynamics that follow neuronal activation. Here, we use fluorescence recovery after photobleaching, biochemical ...

Although tremendous progress has been made toward identifying factors that regulate nucleosome structure and positioning, the mechanisms that regulate higher-order chromatin structure remain poorly understood. Recent studies suggest that the ISWI chromatin-remodeling factor plays a key role in this process by promoting the assembly of chromatin containing histone H1. To test this hypothesis, we...

هرمنوتیک فلسفی یکی از نظریات تفسیر متون در غرب پس رنسانس است که تحولاتی را تفکر معرفتی برخی نواندیشان مسلمان ایجاد نمود. این نظریه افزون بر اختلاف مبانی و اصول، سبب نسبی‌گرایی پلورالیسم تفسیری می‌شود. اندیشمندان حوزة قرآن کریم با غفلت پیامدها پذیرش سعی تطبیق مسئلة پایان‌ناپذیری فهم دارند، بنابراین تحلیل مقایسة دو ضرورت دارد. پژوهش هدف انتقادی روش تحلیلی- تطبیقی درصدد بررسی مؤلفه‌های تفاوت آن اس...

Background Considerable evidence indicates that, in addition to antiallergic effect, several H1-antihistamines also possess antiinflammatory properties. The anti-inflammatory activity of H1 antihistamine treatment in urticaria patients is based on the capacity of H1-antihistamines to inhibit the release of chemical mediators from mast cells and basophiles, to regulate the chemotaxis of neutroph...

PURPOSE Gliomas are known to induce local and systemic immunosuppression, inhibiting T-cell-mediated cytotoxic responses to tumor growth. Tumor-associated macrophages are a significant component of the immune infiltrate in gliomas and may express immunosuppressive surface ligands, such as B7-H1. EXPERIMENTAL DESIGN Tumor and peripheral blood samples from patients with glioblastoma (GBM) were ...