background leptospirosis is a systemic infectious disease caused by pathogenic bacteria belonging to the species of leptospira, it is considered to be the most common zoonosis in the world. this is a serious and sometimes fatal infection which is transmitted from animals to humans by entering the digestive tract or mucosal membrane. within the initial 10-12 day period, the patient may experienc...

actively proliferating. When the cell growth in vivo becomes stationary, sialic acid is added to the galactose on the nonreducing end (17). The antigen molecule was extracted from MM2 cells by hypotonic incubation and was partially purified by sucrose density gradient centrifugation (18). The humoral factor has a specific binding affinity mainly to the saccharide moiety of the antigen molecule....

actively proliferating. When the cell growth in vivo becomes stationary, sialic acid is added to the galactose on the nonreducing end (17). The antigen molecule was extracted from MM2 cells by hypotonic incubation and was partially purified by sucrose density gradient centrifugation (18). The humoral factor has a specific binding affinity mainly to the saccharide moiety of the antigen molecule....

The relative efficacy of the card test in the diagnosis of human brucellosis was evaluated by comparison with four other tests: the standard tube agglutination test, centrifugation agglutination test, 2-mercaptoethanol degradation technique, and 2-mercaptoethanol centrifugation agglutination test. A total of 1,701 serum specimens from persons with various degrees of potential exposure to brucel...

1. Unsensitized sheep cells suspended in sugar solutions are agglutinated by electrolytes whenever the potential is depressed to 6 millivolts or less, except in the case of MgCl(2) or CaCl(2). 2. With these salts no agglutination occurs although there is practically no potential. The presence of these salts prevents acid agglutination. This is presumably due to a decrease in the "cohesion" betw...

When Escherichia coli spheroplasts made by ethylenediaminetetraacetic acid and lysozyme were agglutinated by concanavalin A (con A), the degradation of ribosomal ribonucleic acid (rRNA) was found to occur proportionally to the degree of the agglutination, which was determined by microscopic examination or by a newly devised assay based on the slower settling of aggregates. Methyl-alpha-d-glucos...

We have developed a quantitative assay for tissue-specific adhesive components which is based on the agglutination of glutaraldehyde-fixed cells. At least 2 components are required for fixed-cell agglutination: a cell-surface ligand which is obtained from tissue culture-conditioned medium, and a soluble 'agglutinin' which accumulates in conditioned medium from monolayer cultures. Our results su...

The Wellcolex Colour Salmonella Test was evaluated for detection of Salmonella spp. in enrichment broths of 1,010 stool samples. In 39 specimens, Salmonella spp. could be isolated from the selenite F broth (SF). Wellcolex agglutination indicative of the presence of Salmonella spp. was noted with the SF in 36 cases, 34 of which were in agreement with the subculture results. Therefore, relative t...

Methods to assess immunocompetence requiring only a single sample are useful in comparative studies where practical considerations prevent holding or recapturing individuals. The assay for natural antibody-mediated complement activation and red blood cell agglutination described here, requiring approximately 100 microl of blood, is highly repeatable. The effects of complement deactivation, 2-me...

1. The addition of blood serum displaces the optimum for agglutination of red blood cells in a salt-free medium to the reaction characteristic of flocculation of the serum euglobulin. 2. This effect is not due merely to a mechanical entanglement of the cells by the precipitating euglobulin, since at reactions at which the latter is soluble it protects the cells from the agglutination which occu...