Thermophilic DNA polymerases of the polB family are of great importance in biotechnological applications including high-fidelity PCR. Of particular interest is the relative promiscuity of engineered versions of the exo- form of polymerases from the Thermo- and Pyrococcales families towards non-canonical substrates, which enables key advances in Next-generation sequencing. Despite this there is ...

Administration of high-titre measles vaccine (Edmonston-Zagreb (EZ) at > 10(5) plaque-forming units (PFU) per dose) before the age of 9 months has been recommended in areas with high measles mortality before the routine age of immunization after 9 months. The study compares the long-term survival after high-titre measles immunization at 5 months of age with that following routine immunization w...

هدف: هدف از این مطالعه بررسی اثر سینامیک‌اسید (CA) بر برخی خصوصیات فیزیولوژیکی و میزان تحمل‌به‌شوری گیاه‌تنباکو (Nicotiana rustica L.) می‌باشد.مواد روش‌ها: در قطعات جداکشت گیاه تنباکو رشد‌یافته محیط MS به محیط‌های کشت حاوی (غلظت‌های 0، 5، 10 20 میلی‌گرم‌در‌لیتر) نمک NaCl 100 200 میلی‌مولار) منتقل‌گردید. پس چهار هفته فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز (PAL) تیروزین‌آمونیالیاز (TAL) ، ترکیبات‌فن...

The objective of this study was to evaluate the probability norovirus foodborne illness by raw oyster consumption. One hundred fifty-six samples were collected examine prevalence. inoculated with murine and stored at 4°C-25°C. A plaque assay determined titers. titers fitted Baranyi model calculate shoulder period (h) death rate (Log PFU/g/h). These kinetic parameters a polynomial as function te...

AIM To evaluate the risk factors for breakthrough varicella in a follow up study of a cohort of 181 healthy children immunised when aged 9-24 months with a reformulated Oka strain varicella vaccine (SmithKline Beecham Biologicals/Oka). DESIGN The children were randomised in a double blind manner into one of four groups to receive one of two production lot vaccine batches, at two different tit...

Different amplification protocols were evaluated for use with primer-extension preamplification (PEP). We hypothesized that a protocol known to improve amplification of long DNA fragments would improve efficacy of PEP. Eight DNA samples were preamplified by PCR using different protocols. Treatments consisted of the use of Taq DNA polymerase (T), Taq plus a second polymerase obtained from Pyroco...

BACKGROUND Protection against dengue requires immunity against all 4 serotypes of dengue virus (DENV). Experimental challenge may be useful in evaluating vaccine-induced immunity. METHODS Ten subjects previously vaccinated with a live attenuated tetravalent dengue vaccine (TDV) and 4 DENV-naive control subjects were challenged by subcutaneous inoculation of either 10(3) plaque-forming units (...

Fresh produce is a major vehicle for the transmission of human norovirus (NoV) because it is easily contaminated during both preand post-harvest stages however, the ecology of human NoV in fresh produce is poorly understood. In this study, we determined whether human NoV and its surrogates can be internalized via roots and disseminated to edible portions of the plant. The roots of Romaine lettu...

The genetic diversity of a vesicular stomatitis virus population was analysed by RT-PCR, cloning and sequencing of two approximately 500 nucleotide regions of the virus genome. PCR amplifications were performed in parallel experiments with both Taq and Pfu DNA polymerases, and important differences were observed. Between 10 and 22 mutations were detected when virus populations were analysed by ...

West Nile virus (WNV) and Rift Valley fever virus (RVFV) are two emerging arboviruses transmitted by Culex pipiens species that includes two biotypes: pipiens and molestus. In Lebanon, human cases caused by WNV and RVFV have never been reported. However, the introduction of these viruses in the country is likely to occur through the migratory birds and animal trades. In this study, we evaluated...