Both the adenylylated and unadenylylated forms of Escherichia coli glutamine synthetase catalyze the formation of pyrrolidone carboxylate from glutamate in the presence of ATP and divalent metal ions (Mg++ or Mn++) and in the absence of ammonia. The unadenylylated enzyme catalyzes this reaction more rapidly with Mg++ than with Mn++, while the adenylylated enzyme catalyzes it more rapidly with M...

Reverse transcription in hepadnaviruses is primed by the viral reverse transcriptase (RT) (protein priming) and requires the interaction between the RT and a specific viral RNA template termed epsilon. Protein priming is resistant to a number of RT inhibitors that can block subsequent viral DNA elongation and likely requires a distinct "priming" conformation. Furthermore, protein priming may co...

für Naturforschung in cooperation with the Max Planck Society for the Advancement of Science under a Creative Commons Attribution 4.0 International License. Dieses Werk wurde im Jahr 2013 vom Verlag Zeitschrift für Naturforschung in Zusammenarbeit mit der Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. digitalisiert und unter folgender Lizenz veröffentlicht: Creative Commons Namen...

To reveal what controls the concentration and distribution of possibly hazardous (Mn, U, Se, Cd, Bi, Pb) and nonhazardous (Fe, V, Mo, PO(4)) trace elements in groundwater of the Bengal delta, we mapped their concentrations in shallow groundwater (<60 mbgl) across 102 km(2) of West Bengal. Only Mn is a potential threat to health, with 55% of well water exceeding 0.3 mg/L, the current Indian limi...

The purpose of this study was to evaluate biochemical markers of neurotoxicity following subchronic manganese sulfate (MnSO(4)) inhalation. Juvenile rhesus monkeys were exposed to MnSO(4) at 0, 0.06, 0.3, or 1.5 mg Mn/m(3) for 65 days. Glutamine synthetase (GS), glutamate transporters (glutamate transporter-1 [GLT-1] and glutamate/aspartate transporter [GLAST]) and tyrosine hydroxylase (TH) pro...

I . The minced carcases of twenty-seven lambs, ranging from 18 to 69 kg in live weight, and twenty-five calves (30-90 kg) were analysed for copper, iron, manganese and zinc. The lambs were weaned whereas the calves were reared exclusively on milk. 2. Mean concentrations of Fe, Mn and Zn for groups of lamb carcases fell within the ranges 52.6-75.1, 0.7-1.2 and 20.8-25.6 mg/kg fresh carcase weigh...

A 46-year-old woman showed proteinuria and hematuria after left blepharoptosis, and revealed a histopathology of membranous nephropathy (MN) at renal biopsy. She was diagnosed as having myasthenia gravis (MG) because of a positive edrophonium test and anti-acetylcholine receptor (AchR) antibodies in serum. We found a decrease in anti-AchR antibodies after extended total thymectomy, in parallel ...

Excessive manganese (Mn) exposure is not only a health risk for occupational workers, but also for the general population. Sodium para-aminosalicylic acid (PAS-Na) has been successfully used in the treatment of manganism, but the involved molecular mechanisms have yet to be determined. The present study aimed to investigate the effects of PAS-Na on sub-chronic Mn exposure-induced impairments of...