BACKGROUND Vancomycin-resistant enterococci are pathogenic bacteria that attenuate antibiotic sensitivity by producing peptidoglycan precursors that terminate in D-Ala-D-lactate rather than D-Ala-D-Ala. A key enzyme in effecting antibiotic resistance is the metallodipeptidase VanX, which reduces the cellular pool of the D-Ala-D-Ala dipeptide. RESULTS We constructed eleven mutants, using the r...

The increase in the number and life expectancy of patients with diabetes mellitus (DM) worldwide determines high prevalence late complications diabetes, including diabetic polyneuropathy (DPN), most common type polyneuropathy. Oxidative stress is considered main reason for cellular pathology development mellitus, which use antioxidants DPN treatment. Alpha-lipoic acid (ALA), a natural fat-solub...

This research determines the Acceptable Level of Acceptance (ALA) based on countries with active Nuclear Power Plant (NPP). The ALA is a particular value public acceptance NPP, indicating support and participation in program. If level lower than ALA, then probability resistance against program relatively high would harm NPP. There no correlation between number populations. uses four categories ...

Aminolevulinic acid (ALA) is formed by the enzyme ALA synthase (hemA gene). Then ALA is converted to Porphobilinogen (PBG) by the ALA dehydratase (hemB gene). For the overproduction of ALA, we used an Escherichia coli BL21(DE3) containing a hemA gene from Bradyrhzobium japonicum, which was created in our previous work. The effects of pH on the ALA synthase and ALA dehydratase were investigated....

2 Ser Asn Asn Asn 14 Leu Ile Leu Leu 22 Ser Ser Leu Leu 31 Val Asp Val Val 67 Thr Ala Thr Ala 116 Val Ile Ile Ile 183 Ala Ala Glu Glu 210 Ala Ser Ser Ser 233 Val Ala Ala Ala 262 Thr Ile Thr Thr 277 Gln Gln Arg Gln 294 Thr Val Val Val 296 Pro Ser Ser Ser 305 Ala Ser Ser Ser 314 Ile Val Val Val 317 Glu Lys Lys Lys 378 Ile Leu Leu Leu 415 Leu Val Val Val 417 Asp Gly Gly Gly 421 Val Ala Ala Ala 432...